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Series GSE1904 Query DataSets for GSE1904
Status Public on Oct 29, 2004
Title Maskless photolithography genome tiling experiment
Organism Homo sapiens
Experiment type Expression profiling by genome tiling array
Summary This experiment was designed to identify transcribed sequences across the human genome, particularly those distinct from previously annotated genes. To measure global transcriptional activity a series of high-density oligonucleotide tiling arrays was constructed to represent sense and antisense strands of the entire nonrepetitive sequence of the genome (1.5 Gb). A total of 51,874,388 36mer oligonucleotide probes, positioned every 46 nt on average, were synthesized via maskless photolithography at a feature density of approximately 390,000 probes per slide. The arrays were hybridized to fluorescence-labeled cDNA reverse-transcribed from triple-selected poly (A)+ liver tissue RNA.
Keywords = transcription
Keywords = gene expression
Keywords = human
Keywords: other
 
Web link http://transcriptome.gersteinlab.org
 
Contributor(s) Bertone P
Citation(s) 15539566
Submission date Oct 29, 2004
Last update date Jul 26, 2013
Contact name Paul Bertone
E-mail(s) paul.bertone@yale.edu
Phone (203) 432-5405
Organization name Yale University
Street address
City New Haven
State/province CT
ZIP/Postal code 06520
Country USA
 
Platforms (134)
GPL1539 Human 36mer genome tiling array HS_11648
GPL1540 Human 36mer genome tiling array HS_11650
GPL1541 Human 36mer genome tiling array HS_11662
Samples (134)
GSM34073 Triple-selected poly(A)+ human liver tissue RNA pooled from several individuals (HS_11648)
GSM34075 Triple-selected poly(A)+ human liver tissue RNA pooled from several individuals (HS_11650)
GSM34076 Triple-selected poly(A)+ human liver tissue RNA pooled from several individuals (HS_11662)
Relations
BioProject PRJNA90967

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