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Series GSE190430 Query DataSets for GSE190430
Status Public on Sep 21, 2023
Title Genome-wide Map of R-loop Reveals its interplays with Transcription and Genome Integrity during Germ Cell Meiosis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary R-loop is a three-strand nucleic acid structure composed of mRNA and DNA. In mammalian meiotic cells, R-loop mainly distributed at promoter-related regions, and combined with systematically transcriptome analysis showed R-loop was closely related to transcription in meiotic process. Moreover, knockout of Rnaseh1 in meiotic cells caused mice completely sterile with R-loop accumulation and abnormal DSB repairing in spermatocytes. Further analysis showed R-loop accumulation in leptotene/zygotene influenced transcriptional regulation in pachytene stage. Thus, mutual regulation of R-loop and transcription plays essential role in spermatogenesis.
Overall design A total of 12 samples were analyzed in this study, including WT and Rnaseh1 knock-out spermatogenic cells from two stages, leptotene/zygotene and pachytene. Each biological samples has three replicates.
Contributor(s) Jiang Y, Huang F, Chen L, Wu Y, Jia M, Fan H
Citation(s) 36396044
Submission date Dec 08, 2021
Last update date Sep 21, 2023
Contact name Heng-yu Fan
Organization name Life Sciences Institute
Lab Fan heng-yu
Street address Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM5723694 WT-LZ-1
GSM5723695 WT-LZ-2
GSM5723696 WT-LZ-3
BioProject PRJNA787109
SRA SRP349784

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Supplementary file Size Download File type/resource
GSE190430_Rnaseh1.FPKM.normbyERCC.txt.gz 1.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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