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| Status |
Public on Sep 21, 2023 |
| Title |
Genome-wide Map of R-loop Reveals its interplays with Transcription and Genome Integrity during Germ Cell Meiosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
R-loop is a three-strand nucleic acid structure composed of mRNA and DNA. In mammalian meiotic cells, R-loop mainly distributed at promoter-related regions, and combined with systematically transcriptome analysis showed R-loop was closely related to transcription in meiotic process. Moreover, knockout of Rnaseh1 in meiotic cells caused mice completely sterile with R-loop accumulation and abnormal DSB repairing in spermatocytes. Further analysis showed R-loop accumulation in leptotene/zygotene influenced transcriptional regulation in pachytene stage. Thus, mutual regulation of R-loop and transcription plays essential role in spermatogenesis.
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| Overall design |
A total of 12 samples were analyzed in this study, including WT and Rnaseh1 knock-out spermatogenic cells from two stages, leptotene/zygotene and pachytene. Each biological samples has three replicates.
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| Contributor(s) |
Jiang Y, Huang F, Chen L, Wu Y, Jia M, Fan H |
| Citation(s) |
36396044 |
| |
| Submission date |
Dec 08, 2021 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Heng-yu Fan |
| E-mail(s) |
Fanhengyu_lab@163.com, 21907079@zju.edu.cn
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| Organization name |
Life Sciences Institute
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| Lab |
Fan heng-yu
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| Street address |
Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA787109 |
| SRA |
SRP349784 |
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