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Series GSE190474 Query DataSets for GSE190474
Status Public on Nov 01, 2024
Title CRISPR-Cas9 Whole-Genome Screen Identify Critical Genes Regulating Cholesterol Metabolism in Macrophages
Organism Mus musculus
Experiment type Other
Summary We designed CRISPR-Cas9 whole genome screens to identify and rank critical genes for cholesterol metabolism in modified LDL treated BMDMs
 
Overall design Identify and rank critical genes for cholesterol metabolism in bone marrow-derived macrophages (BMDM) by CRISPR/Cas9 whole genome screen. The screens assayed cellular neutral lipid amounts following exposure of BMDM to low-density lipoprotein. Here, we have screens with change in neutral lipid content following 1) 24-h exposure to AcLDL or OxLDL (24h LDL) 2) 24-h exposure followed by 36-48 h post AcLDL or OxLDL removal culture (post-LDL 48h)
 
Contributor(s) Wanniarachchi K, Sultana R, Wollman J, Huang L, Ge X, Thiex N
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 08, 2021
Last update date Nov 01, 2024
Contact name Natalie W Thiex
E-mail(s) natalie.thiex@sdstate.edu
Phone 605-688-5874
Organization name South Dakota State University
Department Biology & Microbiology
Street address 1390 College Ave
City Brookings
State/province SD
ZIP/Postal code 57006
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (22)
GSM5724292 24-h AcLDL 1: Low fluorescent (Low_LDF1)
GSM5724293 24-h AcLDL 1: High fluorescent (High_LDF1)
GSM5724294 24-h AcLDL 1: Pre-sort (Pre_LDF1)
Relations
BioProject PRJNA787256
SRA SRP349834

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE190474_Counts_24h_AcLDL_1.csv.gz 1.2 Mb (ftp)(http) CSV
GSE190474_Counts_24h_AcLDL_2_deleted.csv.gz 1.6 Kb (ftp)(http) CSV
GSE190474_Counts_24h_AcLDL_2_edited.csv.gz 1.1 Mb (ftp)(http) CSV
GSE190474_Counts_24h_OxLDL_deleted.csv.gz 1.6 Kb (ftp)(http) CSV
GSE190474_Counts_24h_OxLDL_edited.csv.gz 1.1 Mb (ftp)(http) CSV
GSE190474_Counts_No_LDL_deleted.csv.gz 1.6 Kb (ftp)(http) CSV
GSE190474_Counts_No_LDL_edited.csv.gz 929.7 Kb (ftp)(http) CSV
GSE190474_Counts_PostAcLDL_48h_Chase_1.csv.gz 1.2 Mb (ftp)(http) CSV
GSE190474_Counts_PostAcLDL_48h_chase_2.csv.gz 1.1 Mb (ftp)(http) CSV
GSE190474_Counts_PostOxLDL_48h_Chase.csv.gz 1.1 Mb (ftp)(http) CSV
GSE190474_MAGeCK_test_combined.csv.gz 7.3 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record
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