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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Feb 16, 2022 |
| Title |
Collagen10-Cre;Rosa26-tdTomato e16.5 single cell RNA-sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to assess the descendants of hypertrophic chondrocytes, we utilized Collagen10-Cre;Rosa26-tdTomato mouse total bone isolated at e16.5 by Collagenase II digestion after mechanical digestion and soft tissue removal. After sequencing and downstream analysis using Seurat, we observed clusters of cells with gene profiles matching classically defined chondrocytes, skeletal stem and progenitor cells (SSPCs), and osteoblasts. Trajectory analysis reveals that the SSPCs lie intermediate to the transition of chondrocyte to osteoblast. We conclude that hypertrophic chondrocytes dedifferentiate to this progenitor stage before further differentiation.
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| Overall design |
10X Genomics Single cell RNA-sequencing of total bone e16.5 reporter positive cells.
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| Contributor(s) |
Long JT, Leinroth A, Liao Y, Ren Y, Mirando AJ, Nguyen T, Guo W, Sharma D, Rouse D, Wu C, Cheah KS, Karner CM, Hilton MJ |
| Citation(s) |
35179487 |
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| Submission date |
Dec 09, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Matthew J. Hilton |
| E-mail(s) |
matthew.hilton@duke.edu
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| Phone |
9196139761
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| Organization name |
DUKE UNIVERSITY SCHOOL OF MEDICINE
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| Street address |
450 Research Drive, LSRC B321C
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
| GSM5726705 |
Col10a1-Cre;R26-tdTomato total bone cells e16.5 post-FACS |
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| Relations |
| BioProject |
PRJNA787704 |
| SRA |
SRP350159 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE190616_RAW.tar |
151.0 Mb |
(http)(custom) |
TAR (of RDS) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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