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| Status |
Public on Dec 01, 2023 |
| Title |
Gastrointestinal B-cells license metabolic T-cell activation in NASH microbiota/antigen-independently and contribute to fibrosis by IgA-FcRγ signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Aim of the study was to identify transcriptional changes in the intestinal B cells of NASH-affected mice versus healthy mice.
Results: RNA-Seq analysis in both sorted CD19+/CD20+ and sorted B220+ cells, revealed increased expression levels in genes involved in lipid metabolism, and in antigen presentation, BCR signaling, B cells activation and proliferation.
Conclusion: Mice bearing NASH (both WT and μMT choline-deficient high-fat diet (CD-HFD) fed mice) display altered B cells in the gastrointestinal tract towards an activated phenotype and increased proliferation induced by the CD-HFD.
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| Overall design |
RNAseq analysis of sorted CD19+/CD20+ and of B220+ cells from small intestines of WT normal diet (ND), WT CD-HFD, and μMT CD-HFD fed mice for 6-months.
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| Contributor(s) |
Heikenwälder M, Kotsiliti E, Leone V, Unger K, Öllinger R, Rad R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 10, 2021 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Kristian Unger |
| E-mail(s) |
kristian.unger@med.uni-muenchen.de
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| Organization name |
LMU University Hospital
|
| Department |
Department of radiation oncology
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| Lab |
AI-assisted therapy decisions in oncology
|
| Street address |
Marchioninistr. 15
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| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (29)
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| Relations |
| BioProject |
PRJNA787962 |
| SRA |
SRP350295 |
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