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Series GSE190691 Query DataSets for GSE190691
Status Public on Dec 01, 2023
Title Gastrointestinal B-cells license metabolic T-cell activation in NASH microbiota/antigen-independently and contribute to fibrosis by IgA-FcRγ signaling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Aim of the study was to identify transcriptional changes in the intestinal B cells of NASH-affected mice versus healthy mice.
Results: RNA-Seq analysis in both sorted CD19+/CD20+ and sorted B220+ cells, revealed increased expression levels in genes involved in lipid metabolism, and in antigen presentation, BCR signaling, B cells activation and proliferation.
Conclusion: Mice bearing NASH (both WT and μMT choline-deficient high-fat diet (CD-HFD) fed mice) display altered B cells in the gastrointestinal tract towards an activated phenotype and increased proliferation induced by the CD-HFD.
 
Overall design RNAseq analysis of sorted CD19+/CD20+ and of B220+ cells from small intestines of WT normal diet (ND), WT CD-HFD, and μMT CD-HFD fed mice for 6-months.
 
Contributor(s) Heikenwälder M, Kotsiliti E, Leone V, Unger K, Öllinger R, Rad R
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 10, 2021
Last update date Dec 01, 2023
Contact name Kristian Unger
E-mail(s) kristian.unger@med.uni-muenchen.de, kristian.unger@helmholtz-munich.de
Organization name Helmholtz-Zentrum München
Department Research Unit of Radiation Cytogenetics
Lab Integrative Biology
Street address Ingolstädter-Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (29)
GSM5729182 uMT-CDHFD-CD20-BR1
GSM5729183 uMT-CDHFD-CD20-BR2
GSM5729184 uMT-CDHFD-CD20-BR3
Relations
BioProject PRJNA787962
SRA SRP350295

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE190691_157MH11_DGE_Matrix.txt.gz 327.3 Kb (ftp)(http) TXT
GSE190691_215MH16_DGE_Matrix.txt.gz 195.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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