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Series GSE190714 Query DataSets for GSE190714
Status Public on Dec 14, 2021
Title A global timing mechanism regulates cell-type specific wiring programs
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary The assembly of neural circuits is dependent upon precise spatiotemporal expression of cell recognition molecules1–6. Factors controlling cell-type specificity have been identified7–9, but how timing is determined remains unknown. Here we describe the induction of a cascade of transcription factors by a steroid hormone (Ecdysone) in all fly visual system neurons spanning target recognition and synaptogenesis. We demonstrate through single cell sequencing that the Ecdysone pathway regulates the expression of a common set of targets required for synaptic maturation and cell-type specific targets enriched for cell surface proteins regulating wiring specificity. Transcription factors in the cascade regulate the expression of the same wiring genes in complex ways, including activation in one cell-type and repression in another. We show that disruption of the Ecdysone-pathway generates specific defects in dendritic and axonal processes and synaptic connectivity, with the order of transcription factor expression correlating with sequential steps in wiring. We also identify shared targets of a cell-type specific transcription factor and the Ecdysone pathway which regulate specificity. We propose neurons integrate a global temporal transcriptional module with cell-type specific transcription factors to generate different cell-type specific patterns of cell recognition molecules regulating wiring.
Overall design Single cell RNA-seq for 5 developing lamina neurons (L1-L5) in Drosophila visual system with different genetic perturbations (EcRDN, EcR RNAi, and Hr3 RNAi) at multiple time points (24hAPF, 48hAPF, 72hAPF and adult). Bulk RNA-seq and bulk ATAC seq for wild type developing L1 neurons.
Contributor(s) Jain S, Lin Y, Kurmangaliyev YZ, Zipursky SL
Citation(s) 35197627
Submission date Dec 11, 2021
Last update date Mar 17, 2022
Contact name Ying Lin
Organization name University of California, Los Angeles
Street address 675 Charles E. Young Drive South
City Los Angeles
State/province California
ZIP/Postal code 90095
Country USA
Platforms (2)
GPL19132 Illumina NextSeq 500 (Drosophila melanogaster)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
Samples (21)
GSM5729708 bulkATAC-L1-40-1
GSM5729709 bulkATAC-L1-40-2
GSM5729710 bulkATAC-L1-60-1
BioProject PRJNA788151
SRA SRP350367

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Supplementary file Size Download File type/resource
GSE190714_L1_bulk_ATAC-seq_peak_level.xlsx 3.8 Mb (ftp)(http) XLSX
GSE190714_L1_bulk_RNA-seq_expression.xlsx 791.1 Kb (ftp)(http) XLSX
GSE190714_scEcRDN_clustering.rds.gz 34.2 Mb (ftp)(http) RDS
GSE190714_scEcRRNAi_clustering.rds.gz 51.0 Mb (ftp)(http) RDS
GSE190714_scHr3RNAi_clustering.rds.gz 46.1 Mb (ftp)(http) RDS
GSE190714_sclamina_clustering.rds.gz 57.8 Mb (ftp)(http) RDS
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