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| Status |
Public on Dec 15, 2022 |
| Title |
Investigating the role of cell adhesion molecule alpha-catenin during islet development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Alpha catenin mediates cell-cell adhesion through the cadherin-catenin complex. It is important to maintain cell-cell adhesion and tissue organization. In this study we explore how cell-cell adhesion mediated by alpha catenin regulates islet aggregation and analyze the gene expression profiling related with islet adhesion, structure and function.
Methods: We selective ablate alpha catenin (Ctnna1) in endocrine cells of murine pancreas. Total RNA was extracted from pancreatic islets isolated from 8 weeks old mice of Ngn3-Cre; Ctnna1flox/flox and Wild-type controls. There were four animals for each group.
Results: We mapped about 60 million sequence reads per mouse sample. Our results showed that 245 genes were upregulated and 74 were downregulated by alpha catenin ablation.
Conclusions: Our results indicate that alpha catenin is required for normal islet aggregation and cellular composition.
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| Overall design |
Pancreas mRNA profiles of 8-weeks old wild type (WT) and Ngn3-Cre; Ctnna1 flox/flox mice
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| Contributor(s) |
Tixi W, Shih H |
| Citation(s) |
37610090 |
| |
| Submission date |
Dec 13, 2021 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Wenyong Chen |
| Organization name |
City of Hope
|
| Department |
Cancer Biology
|
| Street address |
1500 E. Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA788481 |
| SRA |
SRP350540 |
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