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Status |
Public on Dec 15, 2022 |
Title |
Investigating the role of cell adhesion molecule alpha-catenin during islet development |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: Alpha catenin mediates cell-cell adhesion through the cadherin-catenin complex. It is important to maintain cell-cell adhesion and tissue organization. In this study we explore how cell-cell adhesion mediated by alpha catenin regulates islet aggregation and analyze the gene expression profiling related with islet adhesion, structure and function. Methods: We selective ablate alpha catenin (Ctnna1) in endocrine cells of murine pancreas. Total RNA was extracted from pancreatic islets isolated from 8 weeks old mice of Ngn3-Cre; Ctnna1flox/flox and Wild-type controls. There were four animals for each group. Results: We mapped about 60 million sequence reads per mouse sample. Our results showed that 245 genes were upregulated and 74 were downregulated by alpha catenin ablation. Conclusions: Our results indicate that alpha catenin is required for normal islet aggregation and cellular composition.
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Overall design |
Pancreas mRNA profiles of 8-weeks old wild type (WT) and Ngn3-Cre; Ctnna1 flox/flox mice
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Contributor(s) |
Tixi W, Shih H |
Citation(s) |
37610090 |
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Submission date |
Dec 13, 2021 |
Last update date |
Sep 15, 2023 |
Contact name |
Wenyong Chen |
Organization name |
City of Hope
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Department |
Cancer Biology
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Street address |
1500 E. Duarte Road
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City |
Duarte |
State/province |
CA |
ZIP/Postal code |
91010 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (8)
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Relations |
BioProject |
PRJNA788481 |
SRA |
SRP350540 |