 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 03, 2022 |
| Title |
Next Generation Sequencing Quantitative Analysis of Wild Type and NONO Knock Down Glioblastoma Cell Line(U251) Transcriptomes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified non-POU domain-containing octamer-binding protein (NONO), a Drosophila behavior human splicing (DBHS) protein, among the most upregulated mRNA splicing factors in glioblastoma multiforme (GBM). NONO was associated with poor prognosis in GBM patients, and overexpression of NONO promoted GBM cell proliferation, invasion and tumorigenesis in a GBM orthotopic xenograft model. Through RNA sequencing based transcriptomic profiling, we found that knockdown of NONO resulted in global changes in alternative splicing-intron retention, and identified GPX1 and CCN1 as two pre-mRNAs targeted by NONO. NONO directly bound to the intron of GPX1 pre-mRNA through the RNA-recognition motifs 2 (RRM2) domain and required interaction with another DBHS protein family member, PSPC1. Knockdown of NONO interfered with redox homeostasis in cells, at least partially, through abnormal splicing of GPX1. Finally, Auranofin, a small-molecule inhibitor targeting NONO, inhibited GBM growth in an orthotopic xenograft model in mice. Taken together, our data revealed that NONO was a key regulator of mRNA splicing in GBM, and that targeting NONO represents a novel and effective therapeutic strategy for the treatment of GBM.
|
| |
|
| Overall design |
Glioblastoma cell line U251 mRNA profiles of wild type (siNC) and NONO Knock Down (siNONO) cell line
|
| |
|
| Contributor(s) |
Wang X |
| Citation(s) |
35910786 |
| |
| Submission date |
Dec 15, 2021 |
| Last update date |
Aug 03, 2022 |
| Contact name |
Xu Wang |
| E-mail(s) |
wangxuttxs@gmail.com
|
| Phone |
17865193890
|
| Organization name |
shandong university
|
| Street address |
wenhua west road
|
| City |
jinan |
| State/province |
State... |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA789164 |
| SRA |
SRP351321 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE190950_Processed_Data_Files.txt.gz |
464.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...