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| Status |
Public on Dec 31, 2022 |
| Title |
Reduced representation bisulfite sequencing of equine tissue |
| Organism |
Equus caballus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The aim of the project was to identify the tissue-specific DNA methylation patterns of selected horse tissues, derived from two germ layers, endodermal (liver and lung) and mesenchymal (cardiac striated muscle) origin. The comparative analysis of DNA methylation patterns of the genome fraction rich in CpG dinucleotides was investigated using Reduced Representation Bisulfite Sequencing (RRBS) technique.
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| Overall design |
One-condition experiment, heart vs. lung, heart vs. liver, liver vs. lung. Biological replicates: 4 heart replicates, 4 lung replicates, 4 liver replicates.
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| Contributor(s) |
Semik-Guegul E, Szmatoła T, Gurgul A, Ząbek T |
| Citation missing |
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| |
| Submission date |
Dec 16, 2021 |
| Last update date |
Jan 02, 2023 |
| Contact name |
Ewelina Semik-Gurgul |
| E-mail(s) |
ewelina.semik@iz.edu.pl
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| Organization name |
National Research Institute of Animal Production
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| Department |
Department of Animal Molecular Biology
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| Lab |
Laboratory of Genomics
|
| Street address |
Krakowska 1
|
| City |
Balice |
| ZIP/Postal code |
32083 |
| Country |
Poland |
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| Platforms (1) |
| GPL21401 |
Illumina NextSeq 500 (Equus caballus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA789530 |
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