 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 14, 2022 |
| Title |
Emergence of sex-specific transcriptomes in a sexually-dimorphic brain nucleus |
| Organism |
Taeniopygia guttata |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Sexual dimorphisms in the brain give rise to sex differences in physiology and behavior, yet we have limited understanding of the transcriptomic changes underlying their development. We evaluated developmental transcriptome dynamics for one of the most extreme neuroanatomical sexual dimorphisms in vertebrates - the songbird robust nucleus of the arcopallium (RA). RA is the telencephalic motor output nucleus of the song system. It grows monomorphically for the first few weeks posthatch, then continues growing in males but regresses in females, becoming 5-7 times larger in males. We quantified RA gene expression from monomorphic and dimorphic stages of development in both sexes. Mirroring the morphology, male and female transcriptomes initially resembled one other, then diverged markedly. Thousands of genes showed developmental regulation, corresponding to highly sex-specific biological processes. Males showed enrichments for neuronal growth and morphogenesis, synapse organization, metabolism, and voltage-gated ion channels. Females showed enrichments for cell polarity and differentiation, chemotaxis inhibition, gene silencing, and hormone and immune signaling. Notably, the majority of sex-biased genes in monomorphic RA were sex chromosome Z genes. These findings reveal a profound, sex-specific reorganization of RA’s transcriptome, providing novel insights into potential targets and drivers of sexually dimorphic neurodevelopment.
|
| |
|
| Overall design |
mRNA profiles of brain nucleus RA from 20 DPH and 50 DPH male and female zebra finches
|
| |
|
| Contributor(s) |
Friedrich SR, Nevue AA, Andrade AL, Velho TA, Mello CV |
| Citation(s) |
35926465 |
| |
| Submission date |
Dec 20, 2021 |
| Last update date |
Nov 04, 2022 |
| Contact name |
Claudio V Mello |
| E-mail(s) |
melloc@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Department |
Behavioral Neuroscience
|
| Street address |
3181 SW Sam Jackson Park Rd, L470
|
| City |
Portland |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL27119 |
Illumina NovaSeq 6000 (Taeniopygia guttata) |
|
| Samples (22)
|
|
| Relations |
| BioProject |
PRJNA790926 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE191296_raw_gene_counts_matrix.tsv.gz |
764.4 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...