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Series GSE19226 Query DataSets for GSE19226
Status Public on Dec 10, 2009
Title Genes Differentially Expressed as a result of Candida albicans SC5314 biofilms exposure to 10b
Organism Candida albicans
Experiment type Expression profiling by array
Summary Biomaterial infections are an increasingly alarming problem, and because of their intrinsic recalcitrance to conventional therapy, a new class of antifungal drugs must be explored. 10b, a 2-aminotetralin derivate, was synthesized as a novel chemical structural antifungal agent and exibited strong anti-biofilm activity. To further investigate the action mechanism, we used microarray analysis to investigate the genes expression profiles of C. albicans biofilms treated or untreated with 10b and found 150 genes were differentially expressed. Of them, 69 showed a decrease in expression and 81 showed an increase in expression -10 differentially expressed genes related to biofilm formation, Filamentous or hypha growth. A gene related to specifically hydrolyzing β-1, 3 glucan was significantly increased. 10 down-regulated genes were involved in glycolysis, fermentation and active oxygen scavenging. 15 overexpressed genes were related to the lipid metabolic process. Of them, 13 genes were directly linked to ergosterol biosynthesis including ERG2, ERG6 and ERG11. 10 genes related to translation were over-expressed. Among them, 2 genes involved in negative regulation of transcription were significantly up-regulated.
 
Overall design Total RNA from the control SC5314 biofilms and 10b-treated SC5314 biofilms were used to generate target cDNA, and then hybridized to 8k Candida albicans Genome Array Genechips, representing about 7925 characterized Candida albicans genes. Two independent experiments were conducted. Reference strain was control SC5314 biofilms and test strain was SC5314 biofilms treated with 10b.
 
Contributor(s) Liang R
Citation(s) 20383169
Submission date Nov 30, 2009
Last update date Jun 18, 2019
Contact name Liang Rong-mei
E-mail(s) rmliang2718@163.com
Organization name smmu
Street address Guohe road 325
City Shanghai
ZIP/Postal code 200433
Country China
 
Platforms (1)
GPL9728 28K Candida albicans Genome Array
Samples (2)
GSM476353 Biofilms treated by 10b vs control biofilms 1
GSM476354 Biofilms treated by 10b vs control biofilms 2
Relations
BioProject PRJNA120851

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19226_RAW.tar 6.7 Mb (http)(custom) TAR (of LSR)
Processed data included within Sample table

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