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| Status |
Public on Oct 06, 2023 |
| Title |
Differenatiation of mRNA expression in METTL3-depleted macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
N6-methyladenosine (m6A), the most prevalent internal messenger RNA modification, plays critical roles in diverse biological processes. To characterize the potential involvement of METTL3 in macrophage function, transcriptome sequencing (RNA-seq) was performed in human THP1-derived macrophages with METTL3 depletion. We identified 266 genes that were differentially expressed in METTL3-depleted macrophages (≥ 1.5-fold change, P < 0.05), among which 44% (118 genes) were downregulated and 56% (148 genes) were upregulated. Notably, M2 macrophages activation-associated genes were robustly enhanced in METTL3-depleted macrophages. This study highlights the critical roles of m6A on regulation macrophages homeostasis.
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| Overall design |
The mRNA profiles of WT and METTL3-depleted macrophages under IL-4 stimulation. Three independent experiments were performed at each treatment.
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| Contributor(s) |
Han X, Xiao W |
| Citation(s) |
37957139 |
| |
| Submission date |
Dec 23, 2021 |
| Last update date |
Dec 07, 2023 |
| Contact name |
Xiao Han |
| E-mail(s) |
sqhx12@126.com
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| Phone |
02164932897
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| Organization name |
Children’s Hospital of Fudan University
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| Street address |
399 Wanyuan Rd, Minhang, Shanghai, China
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| City |
Shanghai |
| State/province |
Select state... |
| ZIP/Postal code |
201102 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA791903 |
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