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Series GSE192699 Query DataSets for GSE192699
Status Public on Sep 30, 2022
Title NOMe-Seq analysis of fixed budding yeast nuclei with two DNA methyltransferases
Organism Saccharomyces cerevisiae
Experiment type Methylation profiling by high throughput sequencing
Summary NOMe-Seq analysis was performed with two cytosine C-5 DNA methyltransferases recognizing GC and CC dinucleotides
 
Overall design Spheroplasts were prepared with treating S288C cells with Zymolyase 100T. Then, 1% formaldehyde was added to fix the cells. After washing the cells with 1 M sorbitol, the yeast nuclei were prepared with a solution containing 0.1% NP-40. The nuclei were suspended in methylation buffer, and cytosine C-5 DNA methyltransferase was added. The solution was incubated at 37°C for 1 hr and collected nuclei. Then genomic DNA was extracted, and library preparation was performed with tPBAT protocol (Miura F et al., NAR, 2019)
 
Contributor(s) Miura F
Citation(s) 36333700
Submission date Dec 28, 2021
Last update date Nov 29, 2022
Contact name Fumihito Miura
Organization name The University of Tokyo
Department Graduate School of Frontier Sciences
Street address Kashiwanoha 5-1-5
City Kashiwa
State/province Chiba
ZIP/Postal code 277-8562
Country Japan
 
Platforms (1)
GPL17143 Illumina MiSeq (Saccharomyces cerevisiae)
Samples (2)
GSM5761261 NOMe-Seq, budding yeast treated with GCMT
GSM5761262 NOMe-Seq, budding yeast treated with CCMT
Relations
BioProject PRJNA792790

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE192699_RAW.tar 18.9 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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