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Status |
Public on Sep 30, 2022 |
Title |
NOMe-Seq analysis of fixed budding yeast nuclei with two DNA methyltransferases |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
NOMe-Seq analysis was performed with two cytosine C-5 DNA methyltransferases recognizing GC and CC dinucleotides
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Overall design |
Spheroplasts were prepared with treating S288C cells with Zymolyase 100T. Then, 1% formaldehyde was added to fix the cells. After washing the cells with 1 M sorbitol, the yeast nuclei were prepared with a solution containing 0.1% NP-40. The nuclei were suspended in methylation buffer, and cytosine C-5 DNA methyltransferase was added. The solution was incubated at 37°C for 1 hr and collected nuclei. Then genomic DNA was extracted, and library preparation was performed with tPBAT protocol (Miura F et al., NAR, 2019)
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Contributor(s) |
Miura F |
Citation(s) |
36333700 |
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Submission date |
Dec 28, 2021 |
Last update date |
Nov 29, 2022 |
Contact name |
Fumihito Miura |
Organization name |
The University of Tokyo
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Department |
Graduate School of Frontier Sciences
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Street address |
Kashiwanoha 5-1-5
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City |
Kashiwa |
State/province |
Chiba |
ZIP/Postal code |
277-8562 |
Country |
Japan |
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Platforms (1) |
GPL17143 |
Illumina MiSeq (Saccharomyces cerevisiae) |
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Samples (2) |
GSM5761261 |
NOMe-Seq, budding yeast treated with GCMT |
GSM5761262 |
NOMe-Seq, budding yeast treated with CCMT |
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Relations |
BioProject |
PRJNA792790 |