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Status |
Public on Jan 07, 2022 |
Title |
Endothelial RNF20 regulates the self-renewal and differentiation of neural precursor cells during embryonic development |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: To further explore how endothelial RNF20 regulates embryonic neurogenesis, RNA isolated from the endothelial cells of RNF20fl/fl and RNF20cKO-Tie2 brain cortex at E13 was analyzed by RNA-seq to determine the genome-wide changes. Methods: mRNA from E13 isolated endothelial cells of RNF20fl/fl and RNF20cKO-Tie2 mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, mRNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics Results: Approximately one thousand transcripts showed differential expression between the RNF20fl/fl and RNF20cKO-Tie2 mice brain cortex, with a fold change ≥2 and p value <0.05. Gene ontology analysis of downregulated genes showed the genes were enriched in terms related to neuron differentiation, cell communication and secretion by cells.Concomitantly, upregulated genes were enriched in terms related to negative regulation of cytokine production and cell development.The results showed that endothelial RNF20 is critical for neurogenesis. Conclusions: Endothelial RNF20 RNA-seq would provide a overall understanding how endothelial RNF20 regulates the self-renewal and differentiation of neural precursor cells during embryonic development
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Overall design |
mRNA profiles of E13 RNF20fl/fl and RNF20cKO-Tie2 mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.
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Contributor(s) |
Zhang M, Jiao J |
Citation missing |
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Submission date |
Jan 04, 2022 |
Last update date |
Jan 07, 2022 |
Contact name |
Jianwei Jiao |
Organization name |
Institute of Zoology, Chinese Academy of Sciences
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Department |
State Key Laboratory of Reproductive Biology
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Street address |
1 Beichen West Road
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City |
Beijing |
State/province |
Beijing |
ZIP/Postal code |
100101 |
Country |
China |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (2) |
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Relations |
BioProject |
PRJNA794109 |