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Series GSE192997 Query DataSets for GSE192997
Status Public on Jan 07, 2022
Title Endothelial RNF20 regulates the self-renewal and differentiation of neural precursor cells during embryonic development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: To further explore how endothelial RNF20 regulates embryonic neurogenesis, RNA isolated from the endothelial cells of RNF20fl/fl and RNF20cKO-Tie2 brain cortex at E13 was analyzed by RNA-seq to determine the genome-wide changes.
Methods: mRNA from E13 isolated endothelial cells of RNF20fl/fl and RNF20cKO-Tie2 mice was extracted. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. then, mRNA was converted to cDNA and bound the library. RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics
Results: Approximately one thousand transcripts showed differential expression between the RNF20fl/fl and RNF20cKO-Tie2 mice brain cortex, with a fold change ≥2 and p value <0.05. Gene ontology analysis of downregulated genes showed the genes were enriched in terms related to neuron differentiation, cell communication and secretion by cells.Concomitantly, upregulated genes were enriched in terms related to negative regulation of cytokine production and cell development.The results showed that endothelial RNF20 is critical for neurogenesis.
Conclusions: Endothelial RNF20 RNA-seq would provide a overall understanding how endothelial RNF20 regulates the self-renewal and differentiation of neural precursor cells during embryonic development
 
Overall design mRNA profiles of E13 RNF20fl/fl and RNF20cKO-Tie2 mice were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500.
 
Contributor(s) Zhang M, Jiao J
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Submission date Jan 04, 2022
Last update date Jan 07, 2022
Contact name Jianwei Jiao
Organization name Institute of Zoology, Chinese Academy of Sciences
Department State Key Laboratory of Reproductive Biology
Street address 1 Beichen West Road
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM5770365 4WT
GSM5770366 3ECKO
Relations
BioProject PRJNA794109

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Supplementary file Size Download File type/resource
GSE192997_RNF20ECKO3_RNF20WT4.anno.txt.gz 7.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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