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| Status |
Public on Jul 25, 2022 |
| Title |
CroR-regulated genes in Enterococcus faecalis OG1 |
| Organism |
Enterococcus faecalis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal was to identify genes that are regulated by cell wall stress (bacitracin treatment) in wild-type cells but not in mutant cells lacking CroR, thereby revealing genes of the CroR regulon. Cells of a mutant lacking CroR or its otherwise isogenic wild-type E. faecalis OG1 were cultured in Mueller-Hinton broth and treated (or not) with bacitracin for 15 min. Total RNA was purified, depleted for rRNA, and subjected to preparation of TruSeq stranded mRNA libraries before sequencing on a NextSeq 550 (76-bp single read run).
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| Overall design |
Four replicates per condition (wild-type untreated, wild-type treated, CroR mutant untreated, CroR mutant treated). Cells cultured at 37 C until exponential phase, then treated or not with bacitracin at 128 ug/ml for 15 min before RNA extraction.
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| Contributor(s) |
Atkinson SN, Little JL, Kristich CJ |
| Citation(s) |
35913163 |
| |
| Submission date |
Jan 04, 2022 |
| Last update date |
Sep 15, 2022 |
| Contact name |
CHRIS KRISTICH |
| E-mail(s) |
ckristich@mcw.edu
|
| Organization name |
MCW
|
| Street address |
8701 WATERTOWN PLANK RD
|
| City |
MILWAUKEE |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
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| Platforms (1) |
| GPL31169 |
NextSeq 550 (Enterococcus faecalis) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA794180 |
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