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| Status |
Public on Jan 10, 2022 |
| Title |
A Bacillus anthracis RNA binding protein post-transcriptionally regulates two component signaling through RNA turnover [RIP-Seq] |
| Organism |
Bacillus anthracis |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Formaldehyde cross-linking RNA immunoprecipitation coupled with illumina sequencing (fRIP-seq) was performed to pinpoint the direct RNA targets of KrrA using a FLAG-tagged KrrA construct that is driven by a constitutive promoter Plgt (pOS1.PlgtkrrA-FLAG).
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| Overall design |
Two different growth conditions were implemented: LB and LB supplemented with ‘205. Two controls were included to ensure specificity: (i) a WT strain that does not harbor the FLAG-tagged KrrA construct as a nonspecific background control, and (ii) RNA extracted from WT cells harvested before immunoprecipitation, serving as an input RNA control.
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| Contributor(s) |
Pi H, Skaar EP |
| Citation(s) |
35314695 |
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| Submission date |
Jan 07, 2022 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Hualiang Pi |
| E-mail(s) |
hualiangpi@gmail.com
|
| Organization name |
VUMC
|
| Street address |
1161 21st Avenue South
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL31186 |
Illumina NovaSeq 6000 (Bacillus anthracis) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE193212 |
A Bacillus anthracis RNA binding protein post-transcriptionally regulates two component signaling through RNA turnover |
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| Relations |
| BioProject |
PRJNA795438 |
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