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| Status |
Public on Mar 22, 2023 |
| Title |
IEC intrinsic IL-1R signaling holds dual roles in regulating intestinal homeostasis and inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Introduction: Intestinal epithelial cells (IECs) constitute a critical first line of defense against microbes by providing a physical barrier capable of producing antimicrobial peptides (AMPs) and cytokines. While IECs are known to respond to various microbial signals, the precise upstream cues regulating diverse IEC responses are not clear.
Method: Mouse intestinal crypts were isolated from colon tissue. Crypts were differentiated into 3D spheroids in matrigel by stimulating with LWRN media and growth factors. After differentiation colonoids were stimulated with IL-1b, IL-22, or IL-1b and IL-22 for 12 hours then RNA was isolated for RNA sequencing.
Conclusion: Transcriptional profiling of mColonoids reveals IL-1b synergizes with IL-22 to induced a unique gene profile
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| |
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| Overall design |
mRNA profiles of mouse colonoids stimulated with IL-1b, IL-22 or IL-1b and IL-22 for 12 hours by Illumina sequencing in triplicate.
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| |
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| Contributor(s) |
Pasare C, Overcast G, Meibers H |
| Citation(s) |
36976181 |
| |
| Submission date |
Jan 07, 2022 |
| Last update date |
Jun 21, 2023 |
| Contact name |
Chandrashekhar Pasare |
| E-mail(s) |
Chandrashekhar.pasare@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Immunology
|
| Lab |
Chandrashekhar Pasare
|
| Street address |
250 Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA795516 |
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