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Status |
Public on Jan 14, 2022 |
Title |
ERK-Mediated Phosphorylation of OLA1 Controls Adaptive Mitochondria to Nucleus Communication Regulating Cellular Homeostasis [RNA-seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We found that changes in mitochondrial membrane potential or ROS generation trigger the translocation of OLA1 from mitochondria and cytoplasm to the nucleus, regulated by ERK1-mediated phosphorylation of OLA1 on Ser232/Tyr236. Subsequent phosphorylation of nuclear-OLA1 on Thre325 by ERK2 activates its function as a genetic factor regulating cellular homeostasis by modulating HDAC9 expression. Disruption of OLA1 phosphorylation blocks its nuclear translocation, compromised the expression of nuclear-encoded mitochondrial proteins, DNA damage response, and multiple stress-response-related-genes, leading to cellular dysfunction. Our findings reveal that OLA1 is a crucial component of mitonuclear response regulatory hubs essential for regulating cellular adaptation to stress.
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Overall design |
Examination of the effect of OLA1 knockdown and OLA1 nuclear localization on gene expression in pulmonary endothelial cells.
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Contributor(s) |
Afolayan AJ, Sidlowski P |
Citation missing |
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Submission date |
Jan 09, 2022 |
Last update date |
Dec 16, 2022 |
Contact name |
Adeleye J. Afolayan |
E-mail(s) |
aafolayan@mcw.edu
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Phone |
4149555634
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Organization name |
Medical College of Wisconsin
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Street address |
8701 Watertown Plank Rd., PD278, CRI TBRC, Rm C3278
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City |
Milwaukee |
State/province |
Wisconsin |
ZIP/Postal code |
53226 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (6)
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GSM5792025 |
Pulmonary endothelial cells - ECCTRL_1 RNA-seq |
GSM5792026 |
Pulmonary endothelial cells - ECCTRL_2 RNA-seq |
GSM5792027 |
Pulmonary endothelial cells - ECCTRL_3 RNA-seq |
GSM5792028 |
Pulmonary endothelial cells - ECOLA_SI_1 RNA-seq |
GSM5792029 |
Pulmonary endothelial cells - ECOLA_SI_2 RNA-seq |
GSM5792030 |
Pulmonary endothelial cells - ECOLA_SI_3 RNA-seq |
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This SubSeries is part of SuperSeries: |
GSE193319 |
ERK-Mediated Phosphorylation of OLA1 Controls Adaptive Mitochondria to Nucleus Communication Regulating Cellular Homeostasis |
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Relations |
BioProject |
PRJNA795852 |