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Series GSE193374 Query DataSets for GSE193374
Status Public on Jan 17, 2022
Title Beauvericin (BEA) and enniatin B (ENNB)-induced impairment of mitochondria and lysosomes - potential sources of intracellular reactive iron triggering ferroptosis in Atlantic salmon primary hepatocytes
Organism Salmo salar
Experiment type Expression profiling by high throughput sequencing
Summary Beauvericin (BEA) and enniatin B (ENNB) are emerging mycotoxins frequently detected in plant-based fish feed. With ionophoric properties, they have shown cytotoxic potential in mammalian models. Sensitivity in fish is still largely unknown. Primary hepatocytes isolated from Atlantic salmon (Salmo salar) were used as a model and exposed to BEA and ENNB (0.05 – 10 µM) for 48 hours. Microscopy, evaluation of cell viability, total ATP, total H2O2, total iron content, total Gpx enzyme activity, and RNA sequencing were used to characterize the toxicodynamics of BEA and ENNB. Both mycotoxins became cytotoxic at ≥ 5 µM, causing condensation of the hepatocytes followed by formation of blister-like protrusions on the cell's membrane. RNA sequencing analysis at sub-cytotoxic levels indicated BEA and ENNB exposed hepatocytes to experience increased energy expenditure, elevated oxidative stress, and iron homeostasis disturbances sensitizing the hepatocytes to ferroptosis. The present study provides valuable knowledge disclosing the toxic action of these mycotoxins in Atlantic salmon primary hepatocytes.
 
Overall design Primary hepatocytes were extracted from 6 fish. Isolated hepatocyte from each individual fish were exposed to four concentrations of ennb or bea (0µM, 0.05µM, 0.5µM and 2.5 µM) enabeling to investigate the response in hepatocytes from each individual fish, thus removing noise.
 
Contributor(s) Lie KK, Søderstrøm S, Lundebye A, Søfteland L
Citation(s) 35038498
Submission date Jan 10, 2022
Last update date Apr 19, 2022
Contact name Kai Kristoffer Lie
E-mail(s) kli@hi.no
Organization name Institute of Marine research
Department Feed Safety
Street address Pobox 2029 Nordnes
City Bergen
State/province Hordaland
ZIP/Postal code 5817
Country Norway
 
Platforms (1)
GPL28848 Illumina NextSeq 500 (Salmo salar)
Samples (48)
GSM5799132 sample_100
GSM5799133 sample_101
GSM5799134 sample_102
Relations
BioProject PRJNA795982

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193374_Variable_list_inVitro_AM.txt.gz 4.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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