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| Status |
Public on Feb 01, 2023 |
| Title |
Genome-wide Map of R-loops Reveals its interplays with Transcription and Genome Integrity during Germ Cell Meiosis |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
R-loop is a naturally formed three-strand nucleic acid structure that was reported to take part in multiple biological processes. In our study, we firstly mapped R-loop genomic distribution in mammalian meiotic cells by genome-wide S9.6 CUT & Tag seq. R-loop mainly distributed at promoter-related regions, and combined with systematically transcriptome analysis showed R-loop was closely related to transcription in the meiotic process. Moreover, we verified the conclusion by constructing conditional knockout mice of Rnaseh1, which delete the R-loop endonuclease before meiosis entry. Lack of RNase H1 caused mice completely sterile with R-loop accumulation and abnormal DSB repairing in spermatocytes. Further analysis showed R-loop accumulation in leptotene/zygotene influenced transcriptional regulation in the pachytene stage. Thus, mutual regulation of R-loop and transcription plays an essential role in spermatogenesis.
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| Overall design |
Genome-wide R-loop profiling during spermatogenesis generated by S9.6 CUT&Tag deep sequencing
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| Contributor(s) |
Jiang Y, Huang F, Shen L, Fan H |
| Citation(s) |
36396044 |
| |
| Submission date |
Jan 10, 2022 |
| Last update date |
Sep 21, 2023 |
| Contact name |
Heng-yu Fan |
| E-mail(s) |
Fanhengyu_lab@163.com, 21907079@zju.edu.cn
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| Organization name |
Life Sciences Institute
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| Lab |
Fan heng-yu
|
| Street address |
Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA796035 |
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