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Series GSE193401 Query DataSets for GSE193401
Status Public on Feb 01, 2023
Title Genome-wide Map of R-loops Reveals its interplays with Transcription and Genome Integrity during Germ Cell Meiosis
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary R-loop is a naturally formed three-strand nucleic acid structure that was reported to take part in multiple biological processes. In our study, we firstly mapped R-loop genomic distribution in mammalian meiotic cells by genome-wide S9.6 CUT & Tag seq. R-loop mainly distributed at promoter-related regions, and combined with systematically transcriptome analysis showed R-loop was closely related to transcription in the meiotic process. Moreover, we verified the conclusion by constructing conditional knockout mice of Rnaseh1, which delete the R-loop endonuclease before meiosis entry. Lack of RNase H1 caused mice completely sterile with R-loop accumulation and abnormal DSB repairing in spermatocytes. Further analysis showed R-loop accumulation in leptotene/zygotene influenced transcriptional regulation in the pachytene stage. Thus, mutual regulation of R-loop and transcription plays an essential role in spermatogenesis.
Overall design Genome-wide R-loop profiling during spermatogenesis generated by S9.6 CUT&Tag deep sequencing
Contributor(s) Jiang Y, Huang F, Shen L, Fan H
Citation(s) 36396044
Submission date Jan 10, 2022
Last update date Sep 21, 2023
Contact name Heng-yu Fan
Organization name Life Sciences Institute
Lab Fan heng-yu
Street address Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (15)
GSM5799585 SPG-WT1 (S9.6 CUT&Tag)
GSM5799586 SPG-WT2 (S9.6 CUT&Tag)
GSM5799587 SPG-WT3 (S9.6 CUT&Tag)
BioProject PRJNA796035

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Supplementary file Size Download File type/resource
GSE193401_RAW.tar 239.8 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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