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| Status |
Public on Jan 17, 2022 |
| Title |
Med1 controls effector CD8+ T cell differentiation and survival through mediating the transcriptional regulation of C/EBPβ on T-bet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Effector CD8+ T cells are crucial in adaptive immunity for effective protection against infectious pathogens. The regulatory mechanisms underlying CD8+ effector T cell development are incompletely understood. Here, we defined a critical role of mediator complex subunit 1 (Med1) in controlling effector CD8+ T cell differentiation and survival during acute infections. Mice with Med1 deletion in CD8+ T cells exhibited a significantly impaired effector cell expansion with large reduction of KLRG1+ terminally differentiated and Ly6c+ effector cell populations. Med1 deficiency led to enhanced cell apoptosis and expression of exhausted T cell associated inhibitory receptors (PD-1, Tim3, Lag3 and TIGHT). RNA-Seq analysis revealed the defects of T-bet and Zeb2 mediated transcriptional programs in effector differentiation. Overexpression of T-bet could rescue the Med1-deficient CD8+ effector T cell differentiation and survival. Mechanistically, transcription factor C/EBPβ promoted T-bet expression through interacting with Med1 in effector T cells. Collectively, our findings revealed a novel role of Med1 in regulating effector CD8+ T cell differentiation and survival during immune response.
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| Overall design |
WT and KO CD8+ T cells were sorted from the spleens of recipient mice 7 days after LM-OVA infection in the co-transfer model.
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| Contributor(s) |
Zhang B, Jiao A |
| Citation(s) |
36130132 |
| |
| Submission date |
Jan 13, 2022 |
| Last update date |
Sep 29, 2022 |
| Contact name |
Cangang Zhang |
| E-mail(s) |
cg.zhang20195028@stu.xjtu.edu.cn
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| Phone |
13636810556
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| Organization name |
xian jiaotong university
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| Street address |
Yanta district, xi 'an city, shaanxi province
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| City |
xian |
| State/province |
State... |
| ZIP/Postal code |
710000 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA796966 |
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