NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE193707 Query DataSets for GSE193707
Status Public on Dec 31, 2022
Title Genome-wide expression profiling of mediastinal lymph nodal metastasis of stage IIIA NSCLC patients before neoadjuvant chemotherapy
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Non–small-cell lung cancer (NSCLC) is the leading cause of cancer deaths worldwide due to late diagnosis and lack of effective therapies to treat metastatic disease. About one third of NSCLC patients present at diagnosis with locally advanced cancer (stage IIIA) which metastasizes ipsilateral mediastinal or subcarinal lymph nodes (N2). Neoadjuvant chemotherapy (NACT) followed by surgery is the treatment of choice for resectable N2 disease. However, the majority of patients do not respond to NACT and show persistent lymph nodal metastases and an adverse outcome. Our scant knowledge of the molecular biology of lymph node metastases (LNmets) represents a major obstacle to develop more effective therapies. Here, we investigated gene expression profile (coding and non-coding) of LNmets collected by endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) and mediastinoscopy, in two cohorts of stage IIIA patients before NACT (N=70). We discovered a microRNA expression signature in LNmets predictive of NACT response of N2 patients. Importantly, we unveiled a miR-455-5p/PD-L1 regulatory axis which drives chemotherapy resistance and impact T cell viability in NSCLC. Our data suggests the relevance of LNmets molecular profiling to predict response to NACT likewise presents strong evidences of a miRNA-based mechanism which functionally links chemotherapy response with PD-L1 regulation.
 
Overall design A total of 4 samples were profiled, 2 for each experimental condition.
 
Contributor(s) Bianchi F, Cuttano R
Citation(s) 36587234
Submission date Jan 14, 2022
Last update date Apr 07, 2023
Contact name Fabrizio Bianchi
E-mail(s) f.bianchi@operapadrepio.it
Organization name Fondazione IRCCS Casa Sollievo della Sofferenza
Lab Cancer Biomarkers
Street address Viale Padre Pio 7
City San Giovanni Rotondo (FG)
State/province Outside U.S. & Canada
ZIP/Postal code 71013
Country Italy
 
Platforms (1)
GPL23159 [Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay)
Samples (4)
GSM5819036 LNmet_pN2_POOL1
GSM5819037 LNmet_pN2_POOL2
GSM5819038 LNmet_pN0_POOL1
Relations
BioProject PRJNA797367

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193707_RAW.tar 3.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.