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Status |
Public on Feb 10, 2010 |
Title |
Regulation of alternative splicing by histone modifications |
Organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Alternative splicing of pre-mRNA is a prominent mechanism to generate protein diversity, yet its regulation is poorly understood. Here, we demonstrate a direct role for histone modifications in alternative splicing. We find distinctive histone modification signatures which correlate with splicing outcome in a set of human genes. Modulation of histone modifications causes splice site switching. The mechanism for histone-mediated splice site selection involves a histone mark which is read by a chromatin protein, which in turn recruits a splicing regulator. These results outline an adaptor system for reading of histone marks by the pre-mRNA splicing machinery.
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Overall design |
To obtain an estimate of how many PTB-dependent alternative splicing events are regulated by SET2/MRG15-mediated recruitment of PTB, we carried out a genomewide comparative analysis of alternative splicing in hMSC cells depleted of either SETD2, MRG15 or PTB using specific siRNAs, or mock-depleted using a control siRNA.
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Contributor(s) |
Luco RF, Pan Q, Tominaga K, Blencowe BJ, Pereira-Smith OM, Misteli T |
Citation(s) |
- Luco RF, Pan Q, Tominaga K, Blencowe BJ et al. Regulation of alternative splicing by histone modifications. Science 2010 Feb 19;327(5968):996-1000. PMID: 20133523
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Submission date |
Dec 08, 2009 |
Last update date |
May 15, 2019 |
Contact name |
Qun Pan |
E-mail(s) |
qun.pan@utoronto.ca
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Organization name |
University of Toronto
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Street address |
160 College St.
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City |
Toronto |
ZIP/Postal code |
M5S 3E1 |
Country |
Canada |
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Platforms (1) |
GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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Samples (4)
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Relations |
SRA |
SRP001893 |
BioProject |
PRJNA120419 |