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Series GSE19373 Query DataSets for GSE19373
Status Public on Feb 10, 2010
Title Regulation of alternative splicing by histone modifications
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Alternative splicing of pre-mRNA is a prominent mechanism to generate protein diversity, yet its regulation is poorly understood. Here, we demonstrate a direct role for histone modifications in alternative splicing. We find distinctive histone modification signatures which correlate with splicing outcome in a set of human genes. Modulation of histone modifications causes splice site switching. The mechanism for histone-mediated splice site selection involves a histone mark which is read by a chromatin protein, which in turn recruits a splicing regulator. These results outline an adaptor system for reading of histone marks by the pre-mRNA splicing machinery.
 
Overall design To obtain an estimate of how many PTB-dependent alternative splicing events are regulated by SET2/MRG15-mediated recruitment of PTB, we carried out a genomewide comparative analysis of alternative splicing in hMSC cells depleted of either SETD2, MRG15 or PTB using specific siRNAs, or mock-depleted using a control siRNA.
 
Contributor(s) Luco RF, Pan Q, Tominaga K, Blencowe BJ, Pereira-Smith OM, Misteli T
Citation(s)
  • Luco RF, Pan Q, Tominaga K, Blencowe BJ et al. Regulation of alternative splicing by histone modifications. Science 2010 Feb 19;327(5968):996-1000. PMID: 20133523
Submission date Dec 08, 2009
Last update date May 15, 2019
Contact name Qun Pan
E-mail(s) qun.pan@utoronto.ca
Organization name University of Toronto
Street address 160 College St.
City Toronto
ZIP/Postal code M5S 3E1
Country Canada
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (4)
GSM480870 siControl
GSM480871 siMRG15
GSM480872 siPTB
Relations
SRA SRP001893
BioProject PRJNA120419

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19373_RAW.tar 3.1 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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