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| Status |
Public on Mar 01, 2022 |
| Title |
Downregulation of Neurodevelopmental Gene Expression in iPSC-Derived Cerebral Organoids Upon Infection by Human Cytomegalovirus |
| Organisms |
Homo sapiens; Human betaherpesvirus 5 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the application of bulk RNA sequencing on cerebral organoids infected with HCMV TB40eGFP virus or uninfected, within the infected groups the organoids were sorted into 3 distinct populations prior to sequencing based on GFP expression as a proxy for level of infection. These populations have been denoted GFP (+), GFP Inter, GFP (-), for each sorted population 3 organoids of the same line and from the same differentiation were combined to ensure sufficient cell numbers for bulk sequencing analysis.
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| Overall design |
Examination of the effect of HCMV virus on iPSC derived cerebral organoids.
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| Contributor(s) |
O'Brien BS, Mokry RL, Schumacher ML, Pulakanti K, Rao S, Terhune SS, Ebert AD |
| Citation(s) |
35391828 |
| |
| Submission date |
Jan 15, 2022 |
| Last update date |
Apr 19, 2022 |
| Contact name |
Sridhar Rao |
| E-mail(s) |
sridhar.rao@bcw.edu
|
| Phone |
4149373841
|
| Organization name |
BloodCenter of Wisconsin
|
| Department |
Blood Research Institute
|
| Street address |
8727 Watertown Plank Road
|
| City |
Milwaukee |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL24259 |
Illumina HiSeq 2000 (Homo sapiens; Human betaherpesvirus 5) |
|
| Samples (12)
|
| GSM5820568 |
iPSC derived cerebral organoid - Mock A |
| GSM5820569 |
iPSC derived cerebral organoid - Mock C |
| GSM5820570 |
iPSC derived cerebral organoid - Mock D |
| GSM5820571 |
iPSC derived cerebral organoid - Mock M3_D30 |
| GSM5820572 |
iPSC derived cerebral organoid - Pos 1 |
| GSM5820573 |
iPSC derived cerebral organoid - Pos 2 |
| GSM5820574 |
iPSC derived cerebral organoid - Inter 1 |
| GSM5820575 |
iPSC derived cerebral organoid - Inter 2 |
| GSM5820576 |
iPSC derived cerebral organoid - Inter 3 |
| GSM5820577 |
iPSC derived cerebral organoid - Neg 1 |
| GSM5820578 |
iPSC derived cerebral organoid - Neg 2 |
| GSM5820579 |
iPSC derived cerebral organoid - Neg 3 |
|
| Relations |
| BioProject |
PRJNA797654 |
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