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| Status |
Public on Mar 09, 2022 |
| Title |
SAGA Complex Subunits in Candida albicans Differentially Regulate Filamentation, Invasiveness and Biofilm Formation |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study we were interested in studying the role of SAGA subunits in various cellular processes- morphogenetic changes, growth, invasiveness, biofilm formation and to check the role of these subunits under various cellular and genotoxic conditions. In this work, we investigated conditional and null mutants of components of the SAGA complex modules; Ngg1 of the HAT module, Ubp8 of the Dub module, Tra1 of the recruitment module, Spt7 of the architecture module, and Spt8 of the TBP interaction unit to assess their role in processes such as filamentation, invasiveness, and biofilm formation. Spt7 and Spt8 deletion in Candida albicans resulted in ifilamentatation, invasiveness in YPD media at 30 degrees Celsius and also showed sensitivity to antifungal drugs. Wild type SN148 do not make any filaments in YPD at 30 degrees Celsius and didn't show antifungal sensitivity . The aim was to look for transcription profiling of SAGA mutants against wild type to find genes up and down regulated in the mutant especially those ones critical for filamentation, invasiveness and antifungal drug resistance.
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| Overall design |
Liquid cultures of wild-type (WT) and SAGA subunits were adjusted to OD600 at 0.8 in 10 mL liquid YPD medium and incubated at room temperature for 8 hours with shaking at 220 rpm.
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| Contributor(s) |
Rashid S, Whiteway M |
| Citation(s) |
35350439 |
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| Submission date |
Jan 18, 2022 |
| Last update date |
Apr 06, 2022 |
| Contact name |
Saima Rashid |
| E-mail(s) |
shiekhsaima789@gmail.com
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| Organization name |
Concordia University
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| Department |
Department of Arts and Science
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| Lab |
Whiteway Lab
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| Street address |
7141 Sherbrooke St W
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H4B1R6 |
| Country |
Canada |
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| Platforms (1) |
| GPL23041 |
Illumina MiSeq (Candida albicans) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA798386 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE193907_RAW.tar |
23.2 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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