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| Status |
Public on Feb 01, 2023 |
| Title |
TimeLapse-seq reveals that ALKBH5 modulates RNA stability of metabolic transcripts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
N6-methyadenosine (m6A) RNA modification controls numerous cellular processes through regulation of RNA stability and translational efficiency. Whether the RNA m6A modification regulates energy metabolism process by posttranscriptional regulation is unknown. We here show that loss of the m6A demethylase ALKBH5 results in instability of oxogluatarate-dehydrogenase (Ogdh) messenger RNA and transcripts of other metabolic enzymes.
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| Overall design |
RNA isolated from wildtype and Alkbh5-deficient murine lineage-depleted bone marrow cells treated +/- s4U was subjected to oxidative-nucleophilic-aromatic-substitution chemistry. Sequencing libraries were prepared from all RNA.
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| Contributor(s) |
Gao Y, Zimmer JT, Vasic R, Liu C, Gbyli R, Zheng S, Patel A, Liu W, Nelakanti R, Song Y, Biancon G, Xiao AZ, Slavoff S, Flavell RA, Simon MD, Tebaldi T, Li H, Halene S |
| Citation(s) |
37742191 |
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| Submission date |
Jan 21, 2022 |
| Last update date |
Dec 06, 2023 |
| Contact name |
Matthew Simon |
| E-mail(s) |
matthew.simon@yale.edu
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| Organization name |
Yale University
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| Street address |
300 Heffernan Dr, 300 Hefferna Dr
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| City |
West Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06516 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA799320 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE194148_RAW.tar |
2.0 Gb |
(http)(custom) |
TAR (of BEDGRAPH, CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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