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| Status |
Public on Jan 24, 2022 |
| Title |
Transcriptomic profile of ovarian cancer stem cells dervived from ascites of chickens. |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The objective of the present study was to determine the trancriptomic profile of ovarain cancer stem cells (CSCs) dervived from acites of chickens. Ovarian CSCs were sorted based on presence of a stem cell marker; aldehyde dehydrogenase (ALDH) and then subjected to RNASEq. Pairwise statistical analysis of transcriptomic data using Deseq2 software revealed 1800 differentially expressed (DEGs) in ALDH+ cells as compared to ALDH- cells
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| Overall design |
Ovarian CSCs were seggregated based on the presence of ALDH activity and then sequenced. We performed RNAseq analysis using the Illumina® software platform on RNA samples extracted from ALDH+ and ALDH- cells. On average, 50 million 125 base pair paired-end reads were sequenced for each replicate. Statistical analysis was performed for pairwise comparison between treatment and control group.
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| Contributor(s) |
Tiwari A, Hadley JA, Gopalan L, Ramachandran R, Sebastian A, Albert I, Praul CA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 21, 2022 |
| Last update date |
Jan 24, 2022 |
| Contact name |
Aswathy Sebastian |
| E-mail(s) |
azs13@psu.edu
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| Phone |
5204613475
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| Organization name |
PennState
|
| Street address |
University Park
|
| City |
State College |
| ZIP/Postal code |
16801 |
| Country |
USA |
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| Platforms (1) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA799359 |
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