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| Status |
Public on Nov 30, 2010 |
| Title |
miRNA profiling by Agilent mouse miRNA microarray |
| Platform organism |
Mus musculus |
| Sample organism |
Urocitellus parryii |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
miRNAs are 19-25 nucleotides long small RNAs now well-known for their regulatory roles in the development and diseases through post-transcriptional and translational controls in a wide range of species. Mammalian hibernation is a physiological process involving dramatic metabolic suppression and cellular reorganization, during which miRNAs may play an important role. We systematically analyzed the miRNAs in the liver of an extreme hibernating species, arctic ground squirrels (Spermophilus parryii), during two stages of hibernation compared to non-hibernating animals by massively parallel Illumina sequencing technology. We identified more than 200 ground squirrel miRNAs including novel miRNAs specific to ground squirrel and a fast-evolving miRNA cluster that also showed significant differential expression during hibernation. Integrating with Agilent miRNA microarray and Real-time PCR results, we identified that mir-211, mir-378, mir-184, mir-200a, and mir-320 were significantly under-expressed during hibernation, whereas mir-144, mir-486, mir-451, mir-142-5p, and mir-1 were over-expressed. Analyses of the their target genes suggested that these miRNAs could play an important role to suppress tumor progression and cell growth during hibernation.
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| Overall design |
Three total RNA pools from arctic ground squirrel livers in Early Arousal(EA), Late Topor(LT), and Post-Reproduction(PR) stages were hybridized to three Agilent mouse miRNA microarrays.
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| Contributor(s) |
Liu Y, Hu W, Wang H, Shao C, Yan Z, Zhao S, Yan J |
| Citation(s) |
20442247 |
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| Submission date |
Dec 11, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Wenchao Hu |
| Organization name |
CAS-MPG Partner Institute for Computational Biology
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| Department |
Functional Genomics Group
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| Street address |
320 Yueyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL7732 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE19808 |
miRNA profiling of miRNAs in Hibernating Arctic Ground Squirrels |
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| Relations |
| BioProject |
PRJNA123913 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19427_RAW.tar |
4.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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