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| Status |
Public on Apr 13, 2010 |
| Title |
Differential expression analysis of T. thermophilus HB8 csoR deficient strain in response to copper. |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
We observed the expression profile of the total mRNA in Thermus thermophilus HB8 csrR deficient strain at 0 and 30 min after the addition of CuSO4.
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| Overall design |
Three csoR deficient strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (1 mL) were inoculated into 250 mL of the synthetic medium and then cultivated at 70°C for 8 hours (A600 value being ~0.4). Then the 1.25 mM of CuSO4 was added into the medium, and continued cultivation. Cells were collected at 0 and 30 min time point after the addition of copper ion, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.
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| Contributor(s) |
Agari Y, Sakamoto K, Agari K, Kuramitsu S, Shinkai A |
| Citation(s) |
20395270, 21054499 |
| |
| Submission date |
Dec 16, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
|
| URL |
http://www.srg.harima.riken.jp/
|
| Organization name |
RIKEN Harima Institute
|
| Department |
SPring-8 Center
|
| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL9209 |
[TTHB8401a520105F] Riken Thermus thermophilus HB8 4K mRNA custom array, library Rev.2 |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE19521 |
SuperSeries for the analysis of transcriptional regulation by Thermus themophilus CsoR (TTHA1719) |
| GSE21875 |
SuperSeries for the study of expression pattern analysis of Thermus thermophilus HB8 |
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| Relations |
| BioProject |
PRJNA123941 |
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