NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE195649 Query DataSets for GSE195649
Status Public on Jan 31, 2022
Title The Plasmodium falciparum protein PfMRP1 functions as an influx ABC transporter  
Organisms Plasmodium falciparum 3D7; Plasmodium falciparum Dd2
Experiment type Expression profiling by high throughput sequencing
Summary Adenosine triphosphate (ATP)-binding cassette (ABC) transporters play an important role in mediating solute or drug transport across cellular membranes. Although this class of transporters has been well characterized in diverse organisms little is known about the physiological roles in Plasmodium falciparum, the deadliest malaria parasite species. We studied the Plasmodium falciparum Multidrug Resistance-associated Protein 1 (PfMRP1; PF3D7_0112200), an ABC transporter localized to the parasite plasma membrane, generating genetic disrupted parasites. We demonstrate that parasites with disrupted pfmrp1 are resistant to folate analogs, methotrexate and aminopterin, with antimalarial activity. This phenotype occurs due to reduction in compound accumulation in the parasite cytoplasm. Phylogenetic analysis supports pfmrp1 being distantly related to ABC transporters in other eukaryotes, suggesting an unusual function. We propose that PfMRP1 can act as a solute importer, a function not previously observed in this organism.
 
Overall design Study focused on the impact of truncating the PfMRP1 transporter on P. falciparum physiology and drug response. RNA was extrated after tighly synchronization of parasites of estimatly 28-32h after cycle initiation. One sample was colected for wild-type and knock-out for the two strains in study the 3D7 and Dd2.
 
Contributor(s) Silva M, Calçada C, Osório NS, Baptista V, Thathy V, Teixeira M, Reid AJ, Ferreira PF, Fidock DA, Veiga MI
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 28, 2022
Last update date Jan 31, 2022
Contact name Nuno S. Osório
E-mail(s) nosorio@med.uminho.pt
Organization name ICVS
Street address Campus de Gualtar
City Braga
ZIP/Postal code 4710-057
Country Portugal
 
Platforms (2)
GPL24926 Illumina HiSeq 2500 (Plasmodium falciparum 3D7)
GPL31288 Illumina HiSeq 2500 (Plasmodium falciparum Dd2)
Samples (4)
GSM5842368 3D7_WT
GSM5842369 3D7_KO
GSM5842370 Dd2_WT
Relations
BioProject PRJNA801625

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE195649_3D7_Dd2_PfMRP1KO.xlsx 69.8 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.