NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE19565 Query DataSets for GSE19565
Status Public on Jan 23, 2010
Title Arp2/3 complex mutants show a pronounced lack of hyphal specific gene expression in Candida albicans
Organism Candida albicans
Experiment type Expression profiling by array
Summary Candida albicans is a diploid fungal pathogen lacking a defined complete sexual cycle, and thus has been refractory to standard forward genetic analysis. Instead, transcription profiling and reverse genetic strategies based on Saccharomyces cerevisiae have typically been used to link genes to functions. To overcome restrictions inherent in such indirect approaches, we have investigated a forward genetic mutagenesis strategy based on the UAU1 technology. We screened 4700 random insertion mutants for defects in hyphal development, and linked two new genes (ARP2 and VPS52) to hyphal growth. Deleting ARP2 abolished hyphal formation, generated round and swollen yeast phase cells, disrupted cortical actin patches and blocked virulence in mice. The mutants also showed a global lack of induction of hyphae-specific genes upon the yeast-to-hyphae switch. Surprisingly, both arp2Δ/Δ and arp2Δ/Δarp3Δ/Δ mutants were still able to endocytose FM4-64 and Lucifer Yellow, although as shown by time-lapse movies internalization of FM4-64 was somewhat delayed in mutant cells. Thus the non-essential role of the Arp2/3 complex discovered by forward genetic screening in C. albicans showed that uptake of membrane components from the plasma membrane to vacuolar structures is not dependent on this actin nucleating machinery.
 
Overall design By forward genetic screening, we have identified genes that are essential for hyphal formation. One of the hits is the Arp2/3 complex, which is essential for hyphal formation, but not for viability in Candida albicans. To gain insights into cellular processes affected by disrupting Arp2/3 complex functions, we performed transcriptional profiling under yeast growth conditions (YPD at 30°C for three hours) or hyphal induction (YPD + 10% FBS at 37°C for three hours) and compared transcriptional consequences of deleting ARP2 to MYO5 and SLA2 microarray data sets (Oberholzer et al., 2006).
 
Contributor(s) Epp E, Walther A, Lépine G, Leon Z, Mullick A, Raymond M, Wendland J, Whiteway M
Citation(s) 20141603
Submission date Dec 18, 2009
Last update date Mar 21, 2012
Contact name Elias Epp
E-mail(s) elias.epp@mail.mcgill.ca
Organization name McGill University
Department Biology
Street address 1205 Docteur Penfield
City Montréal
ZIP/Postal code H3A 1B1
Country Canada
 
Platforms (1)
GPL6822 BRI-NRC C. albicans expression microarray
Samples (12)
GSM487651 WT yeast vs WT hyphae (replica 1)
GSM487652 WT yeast vs WT hyphae (replica 2)
GSM487653 WT yeast vs WT hyphae (replica 3)
This SubSeries is part of SuperSeries:
GSE19583 Forward genetics in Candida albicans reveals the Arp2/3 complex is required for hyphal formation, but not endocytosis
Relations
BioProject PRJNA123971

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19565_RAW.tar 17.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.