|
| Status |
Public on Jun 24, 2010 |
| Title |
H2A.Z Mapping During G0/G1 and Mitosis |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report genome wide mapping of the histone variant H2A.Z during G0/G1 and mitosis in T24 bladder cancer cells. The results show that the broad enrichment pattern of H2A.Z near transcription start sites of active genes is maintained during mitosis. Furthermore, using H2A.Z localization to visualize nucleosome positioning near the start site, we see that the +1 nucleosome of active genes shifts upstream to occupy the transcription start sites during mitosis and the nucleosome depleted region is shortened. H2A.Z is also maintained on the -2 nucleosome which also shifts towrds the transcription start site during mitosis, further contributing to the shorteneing of the nucleosome depleted region.
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| |
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| Overall design |
Examination of H2A.Z duing G0/G1 and mitosis in bladder cancer cells
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| |
|
| Contributor(s) |
Kelly T, Jones P, Berman B |
| Citation(s) |
20864037 |
| Submission date |
Dec 18, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Benjamin Berman |
| E-mail(s) |
bberman@usc.edu
|
| Organization name |
University of Southern California
|
| Department |
USC Epigenome Center
|
| Street address |
1450 Biggy St. #G511
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (4)
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| Relations |
| SRA |
SRP002178 |
| BioProject |
PRJNA122475 |
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