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Series GSE195750 Query DataSets for GSE195750
Status Public on Nov 01, 2022
Title Harnessing the Pre-metastatic Niche Macrophages through the Induction of Trained Immunity to control Metastasis.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We demonstrate that a natural compund B-Glucan (WGP) can induce training of macrophages by upregulating the Sphingolipid biosynthesis pathway and more specifically, the accumulation of S1P, resulting in mitochondrial fission in macrophages. These trained macrophages upon re-stimulation with tumor cells or tumor-derived factors showed increased responsiveness by increased pro-inflammatory cytokine production, increased phagocytosis and cytotoxicity. In vivo training of mice with WGP resulted in the training of Lung Interstitial macrophages and controlled the metastasis to the lungs.
Overall design We employed different in vivo models of Lung metastasis to demonstrate that training of the pre-metastatic lung interstitial macrophages with a natural compound, B-Glucan, induces the control of cancer metastasis to the lungs.
Contributor(s) Yan J, Shrestha R, Ding C, Chariker JH, Rouchka EC
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NIH grant(s)
Grant ID Grant title Affiliation Name
P20 GM103436 IDeA Networks of Biomedical Research Excellence in Kentucky UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION MARTHA E BICKFORD
Submission date Jan 30, 2022
Last update date Nov 01, 2022
Contact name Eric Christian Rouchka
Organization name University of Louisville
Department Biochemistry and Molecular Genetics
Lab KY INBRE Bioinformatics Core
Street address 522 East Gray Street
City Louisville
State/province Kentucky
ZIP/Postal code 40292
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM5849630 JY_13_IM_Control_PBS_1
GSM5849631 JY_14_IM_Control_PBS_2
GSM5849632 JY_15_IM_Control_PBS_3
BioProject PRJNA802055

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Supplementary file Size Download File type/resource
GSE195750_GeneMatrix_RawCounts.txt.gz 220.6 Kb (ftp)(http) TXT
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Processed data are available on Series record

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