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| Status |
Public on Nov 01, 2022 |
| Title |
Harnessing the Pre-metastatic Niche Macrophages through the Induction of Trained Immunity to control Metastasis. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We demonstrate that a natural compund B-Glucan (WGP) can induce training of macrophages by upregulating the Sphingolipid biosynthesis pathway and more specifically, the accumulation of S1P, resulting in mitochondrial fission in macrophages. These trained macrophages upon re-stimulation with tumor cells or tumor-derived factors showed increased responsiveness by increased pro-inflammatory cytokine production, increased phagocytosis and cytotoxicity. In vivo training of mice with WGP resulted in the training of Lung Interstitial macrophages and controlled the metastasis to the lungs.
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| Overall design |
We employed different in vivo models of Lung metastasis to demonstrate that training of the pre-metastatic lung interstitial macrophages with a natural compound, B-Glucan, induces the control of cancer metastasis to the lungs.
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| Contributor(s) |
Yan J, Shrestha R, Ding C, Chariker JH, Rouchka EC |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P20 GM103436 |
IDeA Networks of Biomedical Research Excellence in Kentucky |
UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION |
MARTHA E BICKFORD |
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| |
| Submission date |
Jan 30, 2022 |
| Last update date |
Nov 01, 2022 |
| Contact name |
Eric Christian Rouchka |
| E-mail(s) |
eric.rouchka@louisville.edu
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| Organization name |
University of Louisville
|
| Department |
Biochemistry and Molecular Genetics
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| Lab |
KY INBRE Bioinformatics Core
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| Street address |
522 East Gray Street
|
| City |
Louisville |
| State/province |
Kentucky |
| ZIP/Postal code |
40292 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA802055 |
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