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| Status |
Public on Feb 01, 2023 |
| Title |
Wheat transcriptomic responses to long-term feeding by wheat curl mites |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The economic importance of wheat and its contribution to human and livestock diets has been already demonstrated. However, wheat production is impacted by pests that induce yield reductions. Among these pests, wheat curl mite (WCM, Aceria tosichella Keifer) impacts wheat all around the world. WCM are tiny pests that feed within the whorl of developing leaves and prevent the leaves from unfurling by causing leaves curling. The curling of the leaves provides a protective niche for the WCM. Additionally, WCM are also the vector of serious viruses in wheat. Little is known regarding the impact of the WCM on wheat transcriptome, and to date, only one article has been published describing the wheat transcriptomic changes after 1 day of WCM feeding. To better understand the wheat transcriptome variation after long-term feeding by WCM (10 days post infestation (dpi)), we used an RNA-seq approach. We collected leaves uninfested and infested with WCR from two wheat cultivars: Byrd (WCM resistant) and Settler CL (WCM susceptible) at 10 dpi. Our transcriptomic analysis revealed the common and specific transcriptomic variations in WCM resistant and susceptible wheat cultivars, chromosome specific location of the differentially expressed genes, and also identified the gene functions and pathways involved in WCM resistance. Collectively, our study provides important insights on wheat defense mechanisms against WCM after long-term feeding.
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| Overall design |
Whorl mRNA profiles of 10 days after infestation of resistant (Byrd) and susceptible (Settler CL) wheat cultivar
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| Contributor(s) |
Pingault L, Luong T, Louis J, Hein G |
| Citation missing |
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| BioProject |
PRJNA765290 |
| Submission date |
Feb 02, 2022 |
| Last update date |
Feb 01, 2023 |
| Contact name |
Lise Pingault |
| E-mail(s) |
lise.pingault@unl.edu
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| Phone |
4059854042
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| Organization name |
University of Nebraska-Lincoln
|
| Street address |
1700 East Campus Mall, 312F Entomology Hall
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| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68583 |
| Country |
USA |
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| Platforms (1) |
| GPL25409 |
Illumina NovaSeq 6000 (Triticum aestivum) |
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| Samples (12)
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