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Series GSE196046 Query DataSets for GSE196046
Status Public on Mar 02, 2022
Title A mixture of endocrine disrupting chemicals associated with lower birth weight in children induces adipogenesis and DNA methylation changes in human mesenchymal stem cells
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Endocrine disrupting chemicals (EDCs) are man-made compounds that alter functions of the endocrine system. Environmental mixtures of EDCs might have adverse effects on human health, even though their individual concentrations are below regulatory levels of concerns. However, studies identifying and experimentally testing adverse effects of real-life mixtures are scarce. In this study, we aimed at evaluating an epidemiologically identified EDC mixture in an experi-mental setting to delineate its cellular and epigenetic effects. The mixture was established using data from Swedish Environmental Longitudinal Mother and child Asthma and allergy (SELMA) study where it was associated with lower birth weight, an early marker for prenatal metabolic programming. This mixture was then tested for its ability to change metabolic programming of human mesenchymal stem cells. In these cells, we assessed if the mixture induced adipogenesis and genome-wide DNA methylation changes. The mixture increased lipid droplet accumulation already at concentrations corresponding to levels measured in the pregnant women of the SELMA study. Furthermore, we identified differentially methylated regions in genes important for adipogenesis and thermogenesis. This study shows that a mixture reflecting human real-life exposure can induce molecular and cellular changes during development that could underlie adverse outcomes.
 
Overall design hMSCs were treated with 3 concentrations of Mix G1 and control (DMSO), genomic DNA was collected from triplicate samples of hMSCs from both donors, bisulphite converted and standard Illumina EPIC array was run.
 
Contributor(s) Lizunkova P, Engdahl E, Borbély G, Rüegg J
Citation(s) 35216435
Submission date Feb 03, 2022
Last update date Mar 03, 2022
Contact name Polina Lizunkova
E-mail(s) polina.lizunkova@ebc.uu.se
Organization name Uppsala University
Department Organismal Biology
Street address Norbyvägen 18A
City Uppsala
ZIP/Postal code 75236
Country Sweden
 
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (24)
GSM5858505 Donor1_Control_rep1
GSM5858506 Donor1_Control_rep2
GSM5858507 Donor1_Control_rep3
Relations
BioProject PRJNA803156

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE196046_Matrix_signal_intensities_GEO.txt.gz 106.9 Mb (ftp)(http) TXT
GSE196046_RAW.tar 504.2 Mb (http)(custom) TAR (of IDAT)
Processed data included within Sample table

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