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Status |
Public on Mar 02, 2022 |
Title |
A mixture of endocrine disrupting chemicals associated with lower birth weight in children induces adipogenesis and DNA methylation changes in human mesenchymal stem cells |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by genome tiling array
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Summary |
Endocrine disrupting chemicals (EDCs) are man-made compounds that alter functions of the endocrine system. Environmental mixtures of EDCs might have adverse effects on human health, even though their individual concentrations are below regulatory levels of concerns. However, studies identifying and experimentally testing adverse effects of real-life mixtures are scarce. In this study, we aimed at evaluating an epidemiologically identified EDC mixture in an experi-mental setting to delineate its cellular and epigenetic effects. The mixture was established using data from Swedish Environmental Longitudinal Mother and child Asthma and allergy (SELMA) study where it was associated with lower birth weight, an early marker for prenatal metabolic programming. This mixture was then tested for its ability to change metabolic programming of human mesenchymal stem cells. In these cells, we assessed if the mixture induced adipogenesis and genome-wide DNA methylation changes. The mixture increased lipid droplet accumulation already at concentrations corresponding to levels measured in the pregnant women of the SELMA study. Furthermore, we identified differentially methylated regions in genes important for adipogenesis and thermogenesis. This study shows that a mixture reflecting human real-life exposure can induce molecular and cellular changes during development that could underlie adverse outcomes.
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Overall design |
hMSCs were treated with 3 concentrations of Mix G1 and control (DMSO), genomic DNA was collected from triplicate samples of hMSCs from both donors, bisulphite converted and standard Illumina EPIC array was run.
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Contributor(s) |
Lizunkova P, Engdahl E, Borbély G, Rüegg J |
Citation(s) |
35216435 |
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Submission date |
Feb 03, 2022 |
Last update date |
Mar 03, 2022 |
Contact name |
Polina Lizunkova |
E-mail(s) |
polina.lizunkova@ebc.uu.se
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Organization name |
Uppsala University
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Department |
Organismal Biology
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Street address |
Norbyvägen 18A
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City |
Uppsala |
ZIP/Postal code |
75236 |
Country |
Sweden |
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Platforms (1) |
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Samples (24)
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Relations |
BioProject |
PRJNA803156 |