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Series GSE196108 Query DataSets for GSE196108
Status Public on Feb 11, 2022
Title Transcriptome sequencing reveals two subtypes of cortisol-secreting adrenocortical tumors in dogs and identifies CYP26B1 as a potential new therapeutic target
Organism Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary Cushing’s syndrome (CS) is a serious endocrine disorder that is rare in humans, but relatively common in dogs. In ~15-20% of cases, CS is caused by a cortisol-secreting adrenocortical tumor (csACT). To identify differentially expressed genes that can improve prognostic predictions after surgery and represent novel treatment targets, we performed RNA sequencing on csACTs (n=48) and normal adrenal cortices (NACs; n=10) of dogs. A gene was declared differentially expressed when the adjusted P-value was <0.05 and the log2 fold change was >2 or <-2. Between NACs and csACTs, 98 genes were differentially expressed. Based on the principal component analysis (PCA) the csACTs were separated in two groups, of which group 1 had significantly better survival after adrenalectomy (P=0.002) than group 2. Between csACT group 1 and group 2, 77 genes were differentially expressed. One of these, cytochrome P450 26B1 (CYP26B1), was significantly associated with survival in both our canine csACTs and in a publicly available dataset of 33 human cortisol-secreting adrenocortical carcinomas. In the validation cohort, CYP26B1 was also expressed significantly higher (P=0.012) in canine csACTs compared to NACs. In future studies it would be interesting to determine whether CYP26B1 inhibitors could inhibit csACT growth in both dogs and humans.
 
Overall design whole transcriptome sequencing of canine cortisol-secreting adrenocortical tumor and normal adrenal cortices
 
Contributor(s) Sanders K, Kooistra HS, van den Heuvel M, Mokry M, Grinwis GC, van den Dungen NA, van Steenbeek FG, Galac S
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Submission date Feb 04, 2022
Last update date Feb 12, 2022
Contact name Frank van Steenbeek
E-mail(s) f.g.vansteenbeek@uu.nl
Phone 0302532848
Organization name Utrecht University
Street address Yalelaan 104
City Utrecht
State/province Select State/Province
ZIP/Postal code 3584 CM
Country Netherlands
 
Platforms (1)
GPL21400 Illumina NextSeq 500 (Canis lupus familiaris)
Samples (59)
GSM5860094 canine cortisol-secreting adrenocortical tumor 1
GSM5860095 canine cortisol-secreting adrenocortical tumor 2
GSM5860096 canine cortisol-secreting adrenocortical tumor 3
Relations
BioProject PRJNA803356

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Supplementary file Size Download File type/resource
GSE196108_Sanders_raw_counts.txt.gz 1.4 Mb (ftp)(http) TXT
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