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| Status |
Public on Feb 05, 2022 |
| Title |
Real-time quantitative PCR analysis of insulin-producing cells differentiation from canine adipose-derved mesenchymal stem cells |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
We established a useful protocol for generating insulin -producing cells (IPCs) from canine adipose mesenchymal stem cells (cAD-MSCs). IPCs were induced from cAD-MSCs with the three-stepwise protocol. For the first stage , cAD-MSCs was induced to definitive endoderm (DE) with the cooperation of Chir99021 and Activin A for 3 days. For the second stage, pancreatic endocrine (PE) progenitor was induced from DE after treatment with taurine, retinoic acid, FGF2 EGF, TGFβ inhibitor, dorsomorphin, nicotinamide, and DAPT for the next 5 days. In the last stage, the combination of taurine, nicotinamide, Glp-1, forskolin, PI3K inhibitor, and TGFβ inhibitor was used to yield efficiently functional IPCs from PE precursors for 5 days. To indentify differences in the expression pattern of genes related to pancreatic beta cell development, we performed real time quantitative polymerase chain reaction (RT-qPCR).
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| Overall design |
qPCR gene expression profiling of differentiation cells at every stage of induction (day 3, day 5, and day 13) compared to undifferentiated cells.
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| Contributor(s) |
Watchareewan R, Quynh DL |
| Citation(s) |
35650303 |
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| Submission date |
Feb 04, 2022 |
| Last update date |
Jun 08, 2022 |
| Contact name |
QUYNH Dang LE |
| E-mail(s) |
ledangquynhbio@gmail.com
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| Phone |
0933115031
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| Organization name |
FALCUTY OF VETERIANARY, CHULALONGKORN UNIVERSITY
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| Street address |
Phayathai
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| City |
Pathumwan |
| State/province |
Bangkok |
| ZIP/Postal code |
10330 |
| Country |
Thailand |
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| Platforms (1) |
| GPL31907 |
VSCBIC Canine Adipose-Mesenchymal stem cells CF96TM real-time PCR detection system |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA803430 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196118_fold-change_data.txt.gz |
688 b |
(ftp)(http) |
TXT |
| GSE196118_non-normalized_data.txt.gz |
744 b |
(ftp)(http) |
TXT |
| GSE196118_normalized_data.txt.gz |
2.0 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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