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| Status |
Public on Feb 10, 2022 |
| Title |
Transcriptional profiling of the Candida albicans response to the DNA damage stressing agent methyl methanesulfonate |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We explored the global transcription profile in response to MMS in C. albicans through RNAseq assay. Here, we identified 407 genes (log2 fold cut off 1.0, P<0.05) whose levels of transcription were significantly affected under the MMS treatment condition; 207 genes were upregulated, and 200 genes were down-regulated. GO term analysis revealed that a small portion of changed genes showed direct roles in DNA repair progress, but a large number of induced genes are involved in antioxidant responses, and some down-regulated genes are involved in nucleosome packing and nitrogen utilization. In addition, the changed transcription of some DNA repair genes, as well as antioxidants genes, are blocked by deleting RAD53. Taken together, treatment of the DNA damage agent MMS induces a global transcriptional change in C. albicans, but not only DNA damage response genes.
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| Overall design |
We used 0.015% MMS to treat the Candida albicans cells and explored the global transcription profile in response to MMS in C. albicans through RNAseq assay.
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| Contributor(s) |
Feng J |
| Citation missing |
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| |
| Submission date |
Feb 07, 2022 |
| Last update date |
Feb 12, 2022 |
| Contact name |
Jinrong Feng |
| E-mail(s) |
jinrongntu@hotmail.com
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| Phone |
+8651385051746
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| Organization name |
Nantong University
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| Street address |
Qixiu Road
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| City |
Nantong |
| ZIP/Postal code |
226001 |
| Country |
China |
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| Platforms (1) |
| GPL23041 |
Illumina MiSeq (Candida albicans) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA804021 |
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