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| Status |
Public on Mar 21, 2022 |
| Title |
Distinct N6-methyladenosine (m6A) Regulation of Innate Immune Responses During Bacterial Infections II |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
N6-methyladenosine (m6A) is a dynamic post-transcriptional RNA modification influencing all aspects of mRNA biology. Here, we examined cellular m6A epitranscriptomes during P.aeruginosa infection to identify m6A-regulated innate immune response genes with or without ALKBH5 knockdown. We showed that a significant portion of cellular genes including many innate immune response genes underwent m6A modifications in 5’UTR and 3’UTR, from which we identified common and distinct m6A-modified genes under different stimulating conditions.
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| Overall design |
RAW264.7 cells at 4×10^5 cells per mL were infected with P. aeruginosa at 107 per mL and harvested at 0, 2, 4, and 6 h post treatment. RAW264.7 cells with ALKBH5 knockdown were infected with P. aeruginosa 6 hr post infection. MeRIP was performed to enrich m6A methylated mRNA with m6A antibody, and sequenced with corresponding inut RNA.
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| Contributor(s) |
Gao S, Feng J, Sorel O, Chen Y, Lai Z |
| Citation(s) |
35288544 |
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| Submission date |
Feb 07, 2022 |
| Last update date |
Mar 24, 2022 |
| Contact name |
Yidong Chen |
| E-mail(s) |
cheny8@uthscsa.edu
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| Phone |
2105629163
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| Organization name |
UT Health Science Center at San Antonio
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| Department |
Population Health Sciences
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| Street address |
8403 Floyd Curl Drive, MSC 7784
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| City |
San Antonio |
| State/province |
Texas |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (28)
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| GSM5866803 |
NC_elution, repeat 2 |
| GSM5866804 |
si1_ALKBH5_input, repeat 1 |
| GSM5866805 |
si1_ALKBH5_input, repeat 2 |
| GSM5866806 |
si1_ALKBH5_elution, repeat 1 |
| GSM5866807 |
si1_ALKBH5_elution, repeat 2 |
| GSM5866808 |
si2_ALKBH5_input, repeat 1 |
| GSM5866809 |
si2_ALKBH5_input, repeat 2 |
| GSM5866810 |
si2_ALKBH5_elution, repeat 1 |
| GSM5866811 |
si2_ALKBH5_elution, repeat 2 |
| GSM5866812 |
bacteria_0h_Input_1, repeat 1 |
| GSM5866813 |
bacteria_2h_Input_1, repeat 1 |
| GSM5866814 |
bacteria_4h_Input_1, repeat 1 |
| GSM5866815 |
bacteria_6h_Input_1, repeat 1 |
| GSM5866816 |
bacteria_0h_Elution_1, repeat 1 |
| GSM5866817 |
bacteria_2h_Elution_1, repeat 1 |
| GSM5866818 |
bacteria_4h_Elution_1, repeat 1 |
| GSM5866819 |
bacteria_6h_Elution_1, repeat 1 |
| GSM5866820 |
bacteria_0h_Input_1, repeat 2 |
| GSM5866821 |
bacteria_2h_Input_1, repeat 2 |
| GSM5866822 |
bacteria_4h_Input_1, repeat 2 |
| GSM5866823 |
bacteria_6h_Input_1, repeat 2 |
| GSM5866824 |
bacteria_0h_Elution_1, repeat 2 |
| GSM5866825 |
bacteria_2h_Elution_1, repeat 2 |
| GSM5866826 |
bacteria_4h_Elution_1, repeat 2 |
| GSM5866827 |
bacteria_6h_Elution_1, repeat 2 |
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| This SubSeries is part of SuperSeries: |
| GSE196272 |
Distinct N6-methyladenosine (m6A) Regulation of Innate Immune Responses During Bacterial and Viral Infections |
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| Relations |
| BioProject |
PRJNA804131 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196270_RAW.tar |
2.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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