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Series GSE196319 Query DataSets for GSE196319
Status Public on Apr 28, 2022
Title Centromere interactions promote the maintenance of the multipartite genome in Agrobacterium tumefaciens
Organisms Agrobacterium tumefaciens; Bacillus subtilis PY79
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Other
Summary Many pathogens and symbionts contain multipartite genomes, but how they are stably maintained is poorly understood. The plant pathogen, Agrobacterium tumefaciens, contains four replicons. Recent work indicates that their replication origins cluster at cell poles in a manner that depends on their ParB-family centromeric proteins. Here, we provide evidence that centromeric clustering is mediated by interactions between these centromeric proteins. We further show that the disruption of centromere clustering results in the loss of replicons. Our data establish the role of centromeric clustering in multipartite genome maintenance.
 
Overall design Hi-C and ChIP-seq experiments were performed on wild type and mutant cells of Agrobacterium tumefaciens C58 or 15955 growing in LB, ATGN and mutant cells of Bacillus subtilis PY79 growing in rich media.
 
Contributor(s) Wang X
Citation(s) 35536004
Submission date Feb 07, 2022
Last update date Jul 07, 2022
Contact name Xindan Wang
E-mail(s) xindan@iu.edu
Organization name Indiana University at Bloomington
Department Biology
Lab Biology Building 225
Street address 1001 E 3rd St
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
 
Platforms (2)
GPL28115 NextSeq 550 (Bacillus subtilis PY79)
GPL30555 NextSeq 550 (Agrobacterium tumefaciens)
Samples (29)
GSM5870438 443_Wang_HiC_AtWX283_ATGN
GSM5870439 444_Wang_HiC_AtWX110_ATGN
GSM5870440 445_Wang_HiC_AtWX121_ATGN
Relations
BioProject PRJNA804267

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Supplementary file Size Download File type/resource
GSE196319_RAW.tar 261.4 Mb (http)(custom) TAR (of CSV, TXT, ZIP)
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Raw data are available in SRA
Processed data provided as supplementary file
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