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Series GSE196385 Query DataSets for GSE196385
Status Public on Feb 10, 2022
Title Multiple RSV strains infecting HEp-2 and A549 cells reveal cell line-dependent differences in resistance to RSV infection
Organisms Homo sapiens; Respiratory syncytial virus type A; Respiratory syncytial virus type B
Experiment type Expression profiling by high throughput sequencing
Summary A few transformed cell lines and two historic strains have been extensively used to study respiratory syncytial virus (RSV). We report a thorough molecular and cell biological characterization of HEp-2 and A549 cells infected with four strains of RSV representing both major subgroups as well as historic and more contemporary genotypes -- [RSV/A/Tracy (GA1), RSV/A/Ontario (ON), RSV/B/18537 (GB1), RSV/B/Buenos Aires (BA)] -- via measurements of viral replication kinetics and viral gene expression, immunofluorescence-based imaging of gross cellular morphology and cell-associated RSV, and measurements of host response including transcriptional changes and levels of secreted cytokines and growth factors. Our findings strongly suggest 1) the existence of a conserved difference in gene expression between RSV subgroups A and B; 2) the A549 cell line is a more stringent and natural host of replicating RSV than the HEp-2 cell line; and 3) consistent with previous studies, determining the full effects of viral genetic variation in RSV pathogenesis requires model systems as tractable as transformed cell lines but better representative of the human host.
 
Overall design Sequencing of viral genomes and transcriptomes and host transcriptomes in infections of two different cell lines, HEp-2 and A549, by four different RSV strains.
 
Contributor(s) Rajan A, Piedra F, Aideyan L, McBride T, Robertson M, Johnson HL, Aloisio GM, Henke D, Coarfa C, Stossi F, Kumar Menon V, Doddapaneni H, Muzny DM, Javornik Cregeen SJ, Hoffman KL, Petrosino J, Gibbs RA, Avadhanula V, Piedra PA
Citation(s) 35285685
Submission date Feb 08, 2022
Last update date Apr 20, 2022
Contact name Pedro Piedra
E-mail(s) ppiedra@bcm.edu
Organization name Baylor College of Medicine
Department Virol & Micro
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (5)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL31921 Illumina NovaSeq 6000 (Respiratory syncytial virus type A)
GPL31922 Illumina NovaSeq 6000 (Respiratory syncytial virus type B)
Samples (48)
GSM5879898 Epithelial Cell (HEp2) - Mock 24hr
GSM5879899 Epithelial Cell (HEp2) - Mock 48hr
GSM5879900 Epithelial Cell (HEp2) - Mock 72hr
Relations
BioProject PRJNA804555

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE196385_BA_1.09.2020.clean.csv.gz 1.5 Mb (ftp)(http) CSV
GSE196385_GA1_1.09.2020.clean.csv.gz 1.5 Mb (ftp)(http) CSV
GSE196385_GB1_1.09.2020.clean.csv.gz 1.5 Mb (ftp)(http) CSV
GSE196385_ON_1.09.2020.clean.csv.gz 1.5 Mb (ftp)(http) CSV
GSE196385_RAW.tar 1.7 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data are available on Series record
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