|
| Status |
Public on Jun 24, 2022 |
| Title |
Development of ONT-cappable-seq to unravel the transcriptional landscape of Pseudomonas phages |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We developed ONT-cappable-seq, a specialized long-read RNA sequencing technique that allows end-to-end sequencing of primary prokaryotic transcripts using the Nanopore sequencing platform. We applied ONT-cappable-seq to study the transcriptional landscape of Pseudomonas aeruginosa phage LUZ7, leading to a comprehensive genome-wide map of viral transcription start sites, terminators and complex operon structures that fine-regulate gene expression. At the same time, it provides new insights in the RNA biology of LUZ7 and paves the way for more in depth transcription studies that can help unveil the complex layers of phage-host interactions.
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| Overall design |
Full-length transcriptional profiling of P. aeruginosa US449 infected with phage LUZ7 at different time points (0, 5, 10 and 20 minutes) using ONT-cappable-seq.
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| Contributor(s) |
Putzeys L, Boon M, Lammens E, Kuznedelov K, Severinov K, Lavigne R |
| Citation(s) |
35685363, 37092882 |
| |
| Submission date |
Feb 16, 2022 |
| Last update date |
Aug 25, 2023 |
| Contact name |
Rob Lavigne |
| E-mail(s) |
rob.lavigne@kuleuven.be
|
| Organization name |
KU Leuven
|
| Department |
Biosystems
|
| Lab |
Laboratory of Gene Technology
|
| Street address |
Kasteelpark Arenberg 21
|
| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
| |
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| Platforms (1) |
| GPL31946 |
PromethION (Pseudomonas aeruginosa) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA807530 |
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