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| Status |
Public on Jul 01, 2023 |
| Title |
Durable Alterations of Hematopoiesis and Chromatin post-COVID-19 [snATAC-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Acute SARS-CoV-2 infection is often highly inflammatory and protracted. Recent advances have established that inflammation can trigger innate immune memory and a persistent influence on hematopoietic development, through epigenetic mechanisms. However, these phenotypes and their molecular and cellular features are poorly described in humans. Here we reveal epigenomic alterations in innate immune and hematopoietic stem and progenitor cells (HSPC) post-COVID-19, with distinct molecular programs across disease severities. Enabled by novel approaches to study hematopoiesis from peripheral blood, we find persisting HSPC epigenetic programs conveyed, for months to a year, to short-lived progeny monocytes. These epigenetic changes are associated with increased myeloid cell differentiation and inflammatory and antiviral programs. We provide insights into post-infectious HSPC and innate immune cell epigenetic alterations that are broadly relevant.
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| Overall design |
To discover changes in chromatin post-SARS-CoV-2 infection, ATAC-seq was performed on CD34+ HSPCs and CD14+ monocytes sorted from PBMC of healthy, non-COVID-19 post-ICU pateitns, mild convalescent, and severe convalescent COVID-19 patients.
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| Contributor(s) |
Cheong J, Ravishankar A, Sharma S, Parkhurst CN, Josefowicz SZ, Ucar D |
| Citation(s) |
37597510 |
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| Submission date |
Feb 18, 2022 |
| Last update date |
Sep 30, 2023 |
| Contact name |
Jin-Gyu Cheong |
| E-mail(s) |
jic2016@med.cornell.edu
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| Organization name |
Weill Cornell Medicine
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| Street address |
1300 York Avenue
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| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE196990 |
Durable Alterations of Hematopoiesis and Chromatin post-COVID-19 |
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| Relations |
| BioProject |
PRJNA808247 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196989_RAW.tar |
145.0 Mb |
(http)(custom) |
TAR (of RDS, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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