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Series GSE197241 Query DataSets for GSE197241
Status Public on Jun 15, 2022
Title Understanding the underlying molecular mechanisms of meiotic arrest in organotypic culture of prepubertal rat testicular tissues
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary We report the use of the Bulk RNA-sequencing technology on in vivo and in vitro cultured prepubertal testicular tissues. The goal of this study is to identify the origin of the maturation arrest observed in rat prepubertal testicular tissue cultured with the organ culture system with a transcriptomic approach. We compared the gene expression of 14.5 dpp rat testicular tissues cultured up to 6, 16, 22 and 28 days. The statistical filtration revealed 600 differentially expressed genes showing significant differential abundances in all three comparisons. This study uncovered for the first time several mechanisms that could explain the meiotic arrest observed in cultures of rat prepubertal testicular tissues.
 
Overall design Testicular mRNA profiles of 14.5 day old rat and 6, 16, 22 and 28 days cultured tissues
 
Contributor(s) Chalmel F, Saulnier J
Citation(s) 35682573
Submission date Feb 22, 2022
Last update date Jun 15, 2022
Contact name Frédéric Chalmel
E-mail(s) frederic.chalmel@inserm.fr
Organization name Inserm U1085-Irset
Department Physiology and physiopathology of the urogenital tract
Street address 9 avenue du Pr. Léon Bernard
City Rennes
State/province France
ZIP/Postal code 35000
Country France
 
Platforms (1)
GPL22396 Illumina HiSeq 4000 (Rattus norvegicus)
Samples (30)
GSM5911435 InVivo_8.5dpc_1
GSM5911436 InVivo_8.5dpc_2
GSM5911437 InVivo_8.5dpc_3
Relations
BioProject PRJNA809412

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE197241_genes.FPKM.txt.gz 1.7 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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