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| Status |
Public on Feb 26, 2022 |
| Title |
Human and mouse trigeminal ganglia cell atlas implicates multiple cell types in migraine |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The sensitization of trigeminal ganglion neurons contributes to primary headache disorders such as migraine, but the specific neuronal and non-neuronal trigeminal subtypes involved remain unclear. We thus developed a cell atlas in which human and mouse trigeminal ganglia are transcriptionally and epigenomically profiled at single-cell resolution. These data describe evolutionarily conserved and human-specific gene expression patterns within each trigeminal ganglion cell type, as well as the transcription factors and gene regulatory elements that contribute to cell-type-specific gene expression. We then leverage these data to identify trigeminal ganglion cell types that are implicated both by human genetic variation associated with migraine and two mouse models of headache. This trigeminal ganglion cell atlas improves our understanding of the cell types, genes, and epigenomic features involved in headache pathophysiology and establishes a rich resource of cell-type-specific molecular features to guide the development of more selective treatments for headache and facial pain.
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| Overall design |
Single-nucleus RNA sequencing and Single-nucleus ATAC sequencing were performed on trigeminal ganglia from male and female naive mice or two models of headaches (IS or CSD)
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| Contributor(s) |
Yang L, Xu M, Bhuiyan S, Renthal W |
| Citation(s) |
35349784 |
| Submission date |
Feb 23, 2022 |
| Last update date |
May 06, 2022 |
| Contact name |
Lite Yang |
| E-mail(s) |
yanglite1211@gmail.com
|
| Organization name |
Washington University in St. Louis
|
| Street address |
4922 Parkview Place
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
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| Platforms (2) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA809683 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197289_snATAC-seq_human_barcode_meta.csv.gz |
72.0 Kb |
(ftp)(http) |
CSV |
| GSE197289_snATAC-seq_human_raw_counts.RDS.gz |
40.2 Mb |
(ftp)(http) |
RDS |
| GSE197289_snATAC-seq_mouse_barcode_meta.csv.gz |
54.4 Kb |
(ftp)(http) |
CSV |
| GSE197289_snATAC-seq_mouse_raw_counts.RDS.gz |
141.9 Mb |
(ftp)(http) |
RDS |
| GSE197289_snRNA-seq_human_barcode_meta.csv.gz |
822.3 Kb |
(ftp)(http) |
CSV |
| GSE197289_snRNA-seq_human_raw_counts.RDS.gz |
174.0 Mb |
(ftp)(http) |
RDS |
| GSE197289_snRNA-seq_mouse_barcode_meta.csv.gz |
1.9 Mb |
(ftp)(http) |
CSV |
| GSE197289_snRNA-seq_mouse_raw_counts.RDS.gz |
381.1 Mb |
(ftp)(http) |
RDS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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