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| Status |
Public on Mar 08, 2022 |
| Title |
Borrelia burgdorferi, the Lyme disease spirochete, possesses genetically-encoded responses to doxycycline, but not to amoxicillin. |
| Organism |
Borreliella burgdorferi |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To determine whether Borrelia burgdorferi, the spirochetal agent of Lyme disease, responds to sub-lethal doses of antibiotics.
Methods: mRNA profiles of Borrelia burgdorferi were compared after subculturing in 0.2μg/mL amoxicillin, 0.2μg/mL doxycycline, or media control for 3h or 24h (n=3 per group) by paired-end sequencing using the Illumina NextSeq 500. The sequence reads were aligned using Salmon aligner followed by DESeq2 for differential expression analysis.
Results: After culturing for 24 h in a sub-lethal concentration of doxycycline, there were significant increases in a substantial number of transcripts for proteins that are involved with translation. Bacteria continued to elongate, but appeared to be defective in septation and formed filaments. In contrast, incubation with sublethal concentration of amoxicillin led to membrane blebbing and swelling, indicative of cell wall disruption, but no significant changes in levels of any bacterial transcript.
Conclusion: We conclude that B. burgdorferi has a mechanism(s) that detects translational inhibition by doxycycline, and increases production of translational machinery in compensation. In contrast, The absence of response to the inhibition of cell wall synthesis by amoxicillin implies that B. burgdorferi lacks a mechanism to assess cell wall integrity.
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| Overall design |
Borrelia burgdorferi gene expression responses to sub-lethal concentrations of antibiotics.
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| Contributor(s) |
Saylor TC, Casselli T, Brissette CA, Stevenson B |
| Citation(s) |
36178885 |
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| Submission date |
Feb 24, 2022 |
| Last update date |
Oct 26, 2022 |
| Contact name |
Danielle Perley |
| E-mail(s) |
epi.bio.core@und.edu
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| Organization name |
University of North Dakota Genomics Core
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| Street address |
1301 N Columbia Rd
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| City |
Grand Forks |
| State/province |
North Dakota |
| ZIP/Postal code |
58203 |
| Country |
USA |
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| Platforms (1) |
| GPL24990 |
Illumina NextSeq 500 (Borreliella burgdorferi) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA809913 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197338_RAW.tar |
370.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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