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Series GSE197417 Query DataSets for GSE197417
Status Public on Feb 28, 2022
Title Integrated Multiomic Profiling Identifies the Epigenetic Regulator PRC2 as a Therapeutic Target to Counteract Leukemia Immune Escape and Relapse [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary By integrative analysis of gene expression, DNA methylation, and chromatin accessibility in paired diagnosis/relapse primary samples and in the respective patient-derived xenografts (PDXs), we identify the polycomb repressive complex 2 (PRC2) as key epigenetic driver of this immune escape modality. We report that loss of expression of HLA class II molecules is accompanied by a PRC2-dependent reduction in chromatin accessibility. Pharmacological inhibition of PRC2 subunits rescues HLA class II expression in AML relapses in vitro and in vivo, with consequent recovery of leukemia recognition by CD4+ T cells.
 
Overall design Analysis of gene expression profile of 5 relapse post transplantation vs paired diagnostic samples and 5 relapse post chemotherapy vs paired diagnostic samples. 3 relapse post transplantation from PDXs were treated with EPZ-6438 and compared with DMSO controls.
 
Contributor(s) Gambacorta V, Beretta S, Cittaro D, Merelli I, Di Micco R, Vago L
Citation(s) 35255120
Submission date Feb 24, 2022
Last update date May 30, 2022
Contact name Stefano Beretta
E-mail(s) beretta.stefano1@hsr.it
Organization name San Raffaele Telethon Institute for Gene Therapy
Street address Via Olgettina, 60
City Milan
ZIP/Postal code 20132
Country Italy
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (32)
GSM5917121 RNA_AML patient UPN01 at diagnosis
GSM5917122 RNA_AML patient UPN01 at relapse (post transpantation)
GSM5917123 RNA_AML patient UPN02 at diagnosis
This SubSeries is part of SuperSeries:
GSE197419 Integrated Multiomic Profiling Identifies the Epigenetic Regulator PRC2 as a Therapeutic Target to Counteract Leukemia Immune Escape and Relapse
Relations
BioProject PRJNA810181

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE197417_EZH2i_DMSO_RNAseq_counts.tsv.gz 530.6 Kb (ftp)(http) TSV
GSE197417_Primary_PDX_RNAseq_counts.tsv.gz 554.8 Kb (ftp)(http) TSV
GSE197417_Rel_Diagn_CT_RNAseq_counts.tsv.gz 650.8 Kb (ftp)(http) TSV
GSE197417_Rel_Diagn_RNAseq_counts.tsv.gz 651.8 Kb (ftp)(http) TSV
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Processed data are available on Series record

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