NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE197461 Query DataSets for GSE197461
Status Public on Dec 01, 2023
Title Decipher heterogeneity of cervical squamous cell carcinoma and adenocarcinoma with different HPV status: combining scRNA and TCR-seq with 3D organoid
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary Although adenocarcinoma of the cervix (ADC) exhibits a more malignant phenotype and poorer prognosis than squamous cell carcinoma (SCC), they are treated identically. This clinical dilemma calls for deeper investigation into differences between SCC and ADC. We studied 3 human papillomavirus (HPV)-positive SCC, 3 HPV-associated (HPVA) and 2 non-HPV-associated (NHPVA) ADC samples with single-cell RNA and T cell receptor sequencing plus 3D organoid verification. Notably, we revealed the malignant origin of epithelial cells characterized by stemness and poor differentiation and the potential mechanism of HPVA and NHPVA oncogenesis, deciphering differences between SCC and ADC. Additionally, we discovered the key role of tip cells in sprouting angiogenesis, the protumoral effects of cancer-associated fibroblasts and the functional dysregulation of T lymphocytes. We highlighted tumor microenvironment differences between different histologic subtypes through cellular interaction analysis. Importantly, we discovered novel precise treatment strategies specifically for HPV-positive SCC, HPVA and NHPVA-ADC, providing tremendous clinical value.
 
Overall design We performed scRNA-seq and TCR-seq of 8 cervical cancer samples including 3 HPV-positive SCC samples, 3 HPVA ADC samples and 2NHPVA ADC samples
 
Contributor(s) Qiu J, Qu X, Wang L, Tang X, Wang Y, Guo C, Lv B, Jiang Q, Su W, Hua K
Citation(s) 37794698
Submission date Feb 25, 2022
Last update date Dec 02, 2023
Contact name Xinyu Qu
E-mail(s) 14301050218@fudan.edu.cn
Organization name Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University
Department Department of Gynecology
Street address 419 Fangxie Road
City Shanghai
ZIP/Postal code 200011
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (16)
GSM5917937 SCC_1, scRNA-seq
GSM5917938 SCC_2, scRNA-seq
GSM5917939 SCC_3, scRNA-seq
Relations
BioProject PRJNA810439

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE197461_RAW.tar 236.3 Mb (http)(custom) TAR (of CSV, MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.