|
| Status |
Public on Mar 05, 2022 |
| Title |
The Drosophila BEAF Insulator Protein Interacts with the Polybromo Subunit of the PBAP Chromatin Remodeling Complex [Mnase-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We examine the effect of this interaction on gene expression and chromatin structure using precision run-on sequencing (PRO-seq) and micrococcal nuclease sequencing (MNase-seq) after RNAi-mediated knockdown in cultured S2 cells.
We examine the effect of the interaction between BEAF and polybromo on gene expression and chromatin structure using precision run-on sequencing (PRO-seq) and micrococcal nuclease sequencing (MNase-seq) after RNAi-mediated knockdown in cultured S2 cells.
|
| |
|
| Overall design |
Two biological replicates were sampled for each RNAi treatment, LacZ, BEAF, and polybromo. Nuclei were isolated and used for library preparation.
|
| |
|
| Contributor(s) |
McKowen JK, Avva SV, Maharjan M, Duarte FM, Tome JM, Wood JL, Negedu S, Dong Y, Lis JT, Hart CM |
| Citation(s) |
36029240 |
| |
| Submission date |
Feb 28, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Craig Hart |
| E-mail(s) |
keller.mckowen@gmail.com, chart4@lsu.edu
|
| Phone |
225-578-7389
|
| Organization name |
Louisiana State University
|
| Department |
Biological Sciences
|
| Lab |
Hart
|
| Street address |
Life Science Building, LSU
|
| City |
Baton Rouge |
| State/province |
Louisiana |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE197584 |
The Drosophila BEAF Insulator Protein Interacts with the Polybromo Subunit of the PBAP Chromatin Remodeling Complex |
|
| Relations |
| BioProject |
PRJNA811119 |
Less...
More...