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| Status |
Public on Jun 21, 2022 |
| Title |
Altered transcriptional responses in the lungs of aged mice after influenza infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
This study used transcriptomic analysis to evaluate how aging specifically affects the functional host response to influenza in the mouse lung. Adult (12-16 weeks) and aged (72-76 weeks) female BALB/c mice were infected with influenza (A/Puerto Rico/8/34 (H1N1) (A/PR8)) intranasally (instilled with 50 μl of PBS containing 50-100 pfu PR8). Lungs were processed for RNA expression analysis via Agilent microarray at various time points. Time points were 0, 1, 3, 5, 7, 9 days post infection.
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| Overall design |
Factorial experiment. Two mouse age classes: Adult (12-16 weeks); and Aged (72-76 weeks), both tested in six timepoints (0, 1, 3, 5, 7, 9 days post-influenza infection). There were three replicates for each Age x timepoint combination except timepoint = 3 days. In the 3 day treatments, there were two replicates per timepoint. There were a total of 34 samples.
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| Contributor(s) |
Mossman JA, Nau GJ |
| Citation(s) |
35650631 |
| |
| Submission date |
Feb 28, 2022 |
| Last update date |
Jun 22, 2022 |
| Contact name |
Gerard J Nau |
| E-mail(s) |
gerard_nau@brown.edu
|
| Phone |
401-444-7432
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| Organization name |
Alpert School of Medicine at Brown University
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| Department |
Medicine
|
| Street address |
593 Eddy St.
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| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA811138 |
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